|
| Status |
Public on Jan 21, 2014 |
| Title |
Genome wide analysis of genes expressed in early G1 phase of the cell cycle in PMCA4 wild type and PMCA4 knockout primary vascular smooth muscle cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Husain lab cell proliferation in vitro experimental data showed a G1 arrest in PMCA4 knockout primary vascular smooth muscle cells (VSMC). This microarray documents the
different gene expression profiles of PMCA4 wild type and knockout cells at the early G1 stage in serum synchronized cell populations (n=4 populations for each genotype).
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| |
|
| Overall design |
Serum synchronized cell populations were Hoechst labeled and flow sorted for early G1 sub-populations (PMCA4 WT n=4; PMCA4 KO n=4) and total RNA from sorted cells was used for microarray analysis.
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| |
|
| Contributor(s) |
Afroze T, Husain M |
| Citation(s) |
24448801 |
| Submission date |
May 30, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Mansoor Husain |
| E-mail(s) |
mansoor.husain@uhn.ca
|
| Phone |
416-581-7489
|
| Organization name |
University Health Network
|
| Department |
Toronto Gen. Hosp. Res Institute
|
| Lab |
Mansoor Husain lab
|
| Street address |
Room TMDT-3-910, 101 College St.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (8)
|
| GSM938922 |
PMCA4 wild type early G1 replicate 1 |
| GSM938923 |
PMCA4 wild type early G1 replicate 2 |
| GSM938924 |
PMCA4 wild type early G1 replicate 3 |
| GSM938925 |
PMCA4 wild type early G1 replicate 4 |
| GSM938926 |
PMCA4 knockout early G1 replicate 1 |
| GSM938927 |
PMCA4 knockout early G1 replicate 2 |
| GSM938928 |
PMCA4 knockout early G1 replicate 3 |
| GSM938929 |
PMCA4 knockout early G1 replicate 4 |
|
| This SubSeries is part of SuperSeries: |
| GSE38320 |
Regulated Splicing of Plasma Membrane Ca2+ ATPase-4 (PMCA4) Controls Cell Cycle Progression in Vascular Smooth Muscle Cells |
|
| Relations |
| BioProject |
PRJNA167714 |
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