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Series GSE38318 Query DataSets for GSE38318
Status Public on Jan 21, 2014
Title Genome wide analysis of genes expressed in early G1 phase of the cell cycle in PMCA4 wild type and PMCA4 knockout primary vascular smooth muscle cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Husain lab cell proliferation in vitro experimental data showed a G1 arrest in PMCA4 knockout primary vascular smooth muscle cells (VSMC). This microarray documents the
different gene expression profiles of PMCA4 wild type and knockout cells at the early G1 stage in serum synchronized cell populations (n=4 populations for each genotype).
 
Overall design Serum synchronized cell populations were Hoechst labeled and flow sorted for early G1 sub-populations (PMCA4 WT n=4; PMCA4 KO n=4) and total RNA from sorted cells was used for microarray analysis.
 
Contributor(s) Afroze T, Husain M
Citation(s) 24448801
Submission date May 30, 2012
Last update date Jun 14, 2018
Contact name Mansoor Husain
E-mail(s) mansoor.husain@uhn.ca
Phone 416-581-7489
Organization name University Health Network
Department Toronto Gen. Hosp. Res Institute
Lab Mansoor Husain lab
Street address Room TMDT-3-910, 101 College St.
City Toronto
State/province Ontario
ZIP/Postal code M5G 1L7
Country Canada
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM938922 PMCA4 wild type early G1 replicate 1
GSM938923 PMCA4 wild type early G1 replicate 2
GSM938924 PMCA4 wild type early G1 replicate 3
This SubSeries is part of SuperSeries:
GSE38320 Regulated Splicing of Plasma Membrane Ca2+ ATPase-4 (PMCA4) Controls Cell Cycle Progression in Vascular Smooth Muscle Cells
Relations
BioProject PRJNA167714

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38318_RAW.tar 3.1 Mb (http)(custom) TAR
GSE38318_non-normalized.txt.gz 1.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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