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Series GSE38319 Query DataSets for GSE38319
Status Public on Jan 21, 2014
Title Genome wide analysis of genes expressed in late G1/S phase of the cell cycle in PMCA4 wild type (P4WT), PMCA4 knockout(P4KO), P4KO rescued with PMCA4b and P4KO rescued with Vector primary vascular smooth muscle cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Husain lab cell proliferation in vitro experimental data showed that electroporating PMCA4b cDNA into P4KO cells rescued the G1 arrest phenotype in P4KO primary vascular smooth muscle cells (VSMC). This microarray documents the
different gene expression profiles of P4WT, P4KO, P4KO+PMCA4b and P4KO+Vector VSMC at the late G1/S stage in serum synchronized cell populations (n=4 or 5 populations for each genotype).
 
Overall design Serum synchronized late G1/S stage cell populations (P4WT, P4KO, P4KO+PMCA4b and P4KO+Vector) were employed to extract total RNA which was used for microarray analysis.
 
Contributor(s) Afroze T, Husain M
Citation(s) 24448801
Submission date May 30, 2012
Last update date Jun 14, 2018
Contact name Mansoor Husain
E-mail(s) mansoor.husain@uhn.ca
Phone 416-581-7489
Organization name University Health Network
Department Toronto Gen. Hosp. Res Institute
Lab Mansoor Husain lab
Street address Room TMDT-3-910, 101 College St.
City Toronto
State/province Ontario
ZIP/Postal code M5G 1L7
Country Canada
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM938930 PMCA4 wild type late G1/S replicate 1
GSM938931 PMCA4 wild type late G1/S replicate 2
GSM938932 PMCA4 wild type late G1/S replicate 3
This SubSeries is part of SuperSeries:
GSE38320 Regulated Splicing of Plasma Membrane Ca2+ ATPase-4 (PMCA4) Controls Cell Cycle Progression in Vascular Smooth Muscle Cells
Relations
BioProject PRJNA167715

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38319_RAW.tar 3.1 Mb (http)(custom) TAR
GSE38319_non-normalized.txt.gz 4.0 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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