|
Status |
Public on Aug 09, 2012 |
Title |
Transcriptional profiling of lung cancer cells over-expression miR-34a. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
The Zeb1 transcriptional repressor plays a key role in metastasis through the down-regulation of genes that are strong inducers of epithelial differentiation and inhibitors of stem-ness. Here we report that Zeb1 controls the expression of numerous oncogenic and tumor suppressive microRNAs (miRs). Zeb1 stimulated pro-migratory cytoskeletal processes by down-regulating miR-34a and activated Rho GTPases through Arhgap1, a Cdc42 GTPase activating protein and novel miR-34a target gene. Poor-prognosis human lung adenocarcinomas were highly enriched in a cytoskeletal gene signature activated by miR-34a down-regulation. These findings suggest that Zeb1 regulates a miR network and drives pro-migratory cytoskeletal processes through miR-34a.
|
|
|
Overall design |
Total RNA was extracted from primary tumors from mice injected with 344SQ-vector and -miR-34a cells, and then hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array. Determination of differentially expressed genes was carried out.
|
|
|
Contributor(s) |
Creighton C, Kurie J, Ahn Y, Gibbons D |
Citation(s) |
22850877 |
Submission date |
May 30, 2012 |
Last update date |
May 04, 2018 |
Contact name |
Chad Creighton |
E-mail(s) |
creighto@bcm.tmc.edu
|
Organization name |
Baylor College of Medicine
|
Department |
Biostatistics, Ducan Cancer Center
|
Street address |
One Baylor Plaza, Mail Stop: BCM305
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platforms (1) |
GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA167686 |