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| Status |
Public on Nov 08, 2006 |
| Title |
Temporal expression profile of megakaryocytic differentiation primary CD34+ cell culture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of Mk differentiation human CD34-positive hematopoietic stem and progenitor cells cultured with thrombopoietin, interleukin-3, and Flt3-ligand. The goal this study was to identify genes involved in the various facets of Mk differentiation including commitment, polyploidization, proplatelet formation, and apoptosis.
Keywords: time course
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| Overall design |
G-CSF mobilized peripheral blood CD34-positive cells were cultured with TPO, IL-3, and Flt3-L to induce Mk differentiation. Samples prior to day 5, including uncultured starting cells, were analyzed directly, whereas samples after and including day 5 were positively selected for CD41a expression immediately prior to RNA isolation. Three biological replicate experiments were analyzed and approximately one-half of the samples from each experiment were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference RNA pool labeled with Cy5.
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| |
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| Contributor(s) |
Chen C, Fuhrken PG, Miller WM, Papoutsakis ET |
| Citation(s) |
17309828 |
| Submission date |
Dec 15, 2005 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Eleftherios Terry Papoutsakis |
| E-mail(s) |
papoutsakis@dbi.udel.edu
|
| Organization name |
University of Delaware
|
| Department |
Chemical Engineering
|
| Street address |
15 Innovation Way
|
| City |
Newark |
| State/province |
DE |
| ZIP/Postal code |
19711 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL887 |
Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version) |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE8914 |
CHRF-288-11 and primary human megakaryocytic cell cultures provide novel insights into lineage-specific differentiation |
|
| Relations |
| BioProject |
PRJNA104987 |
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