|
| Status |
Public on Oct 30, 2014 |
| Title |
Analysis of blood myelomonocytic cells from RCC patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Myelomonocytic cells (i.e., monocytes or macrophages) play a key role in tumor progression as revealed by numerous mice tumor model studies. However, their contribution in human tumor progression is not well-studied. Using Renal Cell Carcinoma (RCC) as a model for human cancer, we performed a transcriptomal profiling of blood monocytes from RCC patients to investigate the contribution of these cells in cancer progression. As compared to monocytes from healthy donors (Mo), transcriptome analysis of monocytes from RCC patients (RCC-Mo) showed a distinct gene expression signature consisting of cytokines, chemokines and various inflammation-related genes. Validation of these genes by qPCR as well as other functional assays indicated RCC-Mo possess an inflammatory and tumor promoting phenotype.
|
| |
|
| Overall design |
Blood monocytes from RCC patients (with early or metastatic disease) or healthy donors (controls) were isolated, and then differential gene expression was detected with Limma.
|
| |
|
| Contributor(s) |
Biswas SK, Poidinger M |
| Citation(s) |
25453823 |
| Submission date |
Jun 03, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Michael Poidinger |
| E-mail(s) |
michael_poidinger@immunol.a-star.edu.sg
|
| Phone |
+6564070528
|
| URL |
http://www.sign.a-star.edu.sg
|
| Organization name |
Singapore Immunology Network
|
| Department |
Bioinformatics
|
| Street address |
#04-06, 8A Biomedical Grove
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA167836 |
Less...
More...