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| Status |
Public on May 28, 2015 |
| Title |
Enhancing Mammary Differentiation by Overcoming Lineage Specific Epigenetic Modification and Signature Gene Expression of Fibroblast Derived iPSCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Recent studies showed that Induced pluripotent stem cells (iPSCs) could hold memory of their origin and exhibit skewed differentiation potential. This finding reveals a severe limit for the application of iPSCs in cell-based therapy in case certain cell types are not available for reprograming from patients. Here we show that under a typical condition for mammary differentiation, iPSCs derived from mouse mammary epithelium cells (ME-iPSCs) exhibit mammary signature gene expression and chromatin epigenetic modification, leading to smooth progress for mammary gland formation. In contrast, iPSCs reprogramed from tail fibroblasts (TF-iPSCs) displayed fibroblast specific signature that is not compatible for mammary differentiation both in vitro and in vivo. Strikingly, when co-culturing with ME-iPSCs or under pregnant condition, the fibroblast specific signature of TF-iPSCs was erased and the cells gained enhanced ability for mammary differentiation. These findings provide new insights into the precise control of differentiation conditions for future personalized cell-based therapy.
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| |
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| Overall design |
Microarray analysis of three cell types with three biological replications.
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| |
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| Contributor(s) |
Li Y, Zhang A, Chen W, Wang R, Xu X, Deng C |
| Citation(s) |
25476898 |
| Submission date |
Jun 04, 2012 |
| Last update date |
Aug 27, 2015 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10740 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [probe set (exon) version] |
|
| Samples (9)
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| Relations |
| BioProject |
PRJNA168028 |
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