 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 26, 2013 |
| Title |
Human PA-1 cells treated with scrambled or specific ASOs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We describe the discovery of sno-lncRNAs, a class of nuclear-enriched intron-derived long noncoding RNAs (lncRNAs) that are processed on both ends by the snoRNA machinery. During exonucleolytic trimming, the sequences between the snoRNAs are not degraded, leading to the accumulation of lncRNAs flanked by snoRNA sequences but lacking 5' caps and 3' poly(A) tails. Such RNAs are widely expressed in cells and tissues and can be produced by either box C/D or box H/ACA snoRNAs. Importantly, the genomic region encoding one abundant class of sno-lncRNAs (15q11-q13) is specifically deleted in Prader-Willi Syndrome (PWS). The PWS region sno-lncRNAs do not colocalize with nucleoli or Cajal bodies, but rather accumulate near their sites of synthesis. These sno-lncRNAs associate strongly with Fox family splicing regulators and alter patterns of splicing. These results thus implicate a previously unannotated class of lncRNAs in the molecular pathogenesis of PWS.
|
| |
|
| Overall design |
We have used deep sequencing to explore the gene expression from poly(A)+ RNAs in embryonal carcinoma (EC) line PA-1 cells treated with scrambled or specific antisense oligodeoxynucleotides (ASOs).
|
| |
|
| Contributor(s) |
Chen LL, Yin QF, Yang L, Zhang Y, Xiang JF, Wu YW, Carmichael GG |
| Citation(s) |
22959273 |
| Submission date |
Jun 06, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Ling-Ling Chen |
| E-mail(s) |
linglingchen@sibcb.ac.cn
|
| Organization name |
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
|
| Street address |
320 Yue-Yang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA200086 |
| SRA |
SRP021524 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38541_RAW.tar |
471.0 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
