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| Status |
Public on Jan 01, 2013 |
| Title |
Genome-wide maps of H3K27ac localization in HUVECs |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
H3K27Ac is one of the expressed enhancer markers in endothelial cells, but its genomic localization is unknown. This time, we established a new antibody for H3K27ac, and performed ChIP-seq to identify H3K27ac binding site in whole genome manner under hypoxia.
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| Overall design |
We used chromatin immunoprecipitation with deep sequencing (ChIP-seq) of HUVECs treated with or without hypoxia (1%O2) for 24hours, then H3K27ac binding regions were identified. Normoxia was used as a control condition. HUVECs were used within the first 6 passages.
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| Contributor(s) |
Inoue T, Kohro T, Wada Y, Taguchi A, Yamamoto S, Kodama T |
| Citation(s) |
24721177 |
| Submission date |
Jun 07, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Takahide Kohro |
| E-mail(s) |
kohro@lsbm.org
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| Phone |
81-3-5452-5237
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| Organization name |
LSBM, RCAST
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| Street address |
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| City |
4-6-1, Komaba, Meguroku |
| State/province |
Tokyo |
| ZIP/Postal code |
153-8904 |
| Country |
Japan |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA168216 |
| SRA |
SRP013590 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38555_RAW.tar |
301.2 Mb |
(http)(custom) |
TAR (of BED, WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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