|
| Status |
Public on Jun 08, 2012 |
| Title |
Genome-wide profiling of target genes for the systemic lupus erythematosus-associated transcription factors IRF5 and STAT4 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
IRF5 and STAT4 are strongly associated with human systemic lupus erythematosus (SLE). By performing chromatin immunoprecipitation-sequencing (ChIP-Seq) in human peripheral blood mononuclear cells (PBMCs), we identified more than 7000 target genes for IRF5 and STAT4 in stimulated PBMCs. Contrarily, without stimulation IRF5 seemed to be inactive, and STAT4 only showed low levels of transcriptional regulatory activity.
|
| |
|
| Overall design |
Target genes of IRF5 and STAT4 were identified in human PBMCs with or without stimulation using ChIP-Seq.
|
| |
|
| Contributor(s) |
Wang C, Sandling JK, Hagberg N, Berggren O, Sigurdsson S, Karlberg O, Rönnblom L, Eloranta M, Syvänen A |
| Citation(s) |
22730365 |
| Submission date |
Jun 07, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Chuan Wang |
| E-mail(s) |
chuan.wang@medsci.uu.se
|
| Phone |
+46 18 611 2524
|
| Fax |
+ 46 18 55 3601
|
| Organization name |
Uppsala University
|
| Department |
Medical Sciences
|
| Lab |
Molecular Medicine
|
| Street address |
The University Hospital, Entrance 70, 3rd Floor, Research Department II
|
| City |
Uppsala |
| ZIP/Postal code |
SE-751 85 |
| Country |
Sweden |
| |
|
| Platforms (3) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (16)
|
| GSM945598 |
Blood Donor No.1_non-stimulated PBMCs_IRF5 ChIP-Seq |
| GSM945599 |
Blood Donor No.2_non-stimulated PBMCs_IRF5 ChIP-Seq |
| GSM945600 |
Blood Donor No.2_non-stimulated PBMCs_IRF5 ChIP-Seq rep |
| GSM945601 |
Blood Donor No.3_non-stimulated PBMCs_IRF5 ChIP-Seq |
| GSM945602 |
Blood Donor No.4_stimulated PBMCs_IRF5 ChIP-Seq |
| GSM945603 |
Blood Donor No.5_stimulated PBMCs_IRF5 ChIP-Seq |
| GSM945604 |
Blood Donor No.5_stimulated PBMCs_IRF5 ChIP-Seq rep |
| GSM945605 |
Blood Donor No.6_stimulated PBMCs_IRF5 ChIP-Seq |
| GSM945606 |
Blood Donor No.1_non-stimulated PBMCs_STAT4 ChIP-Seq |
| GSM945607 |
Blood Donor No.2_non-stimulated PBMCs_STAT4 ChIP-Seq |
| GSM945608 |
Blood Donor No.3_non-stimulated PBMCs_STAT4 ChIP-Seq |
| GSM945609 |
Blood Donor No.4_stimulated PBMCs_STAT4 ChIP-Seq |
| GSM945610 |
Blood Donor No.5_stimulated PBMCs_STAT4 ChIP-Seq |
| GSM945611 |
Blood Donor No.5_stimulated PBMCs_STAT4 ChIP-Seq rep |
| GSM945612 |
Blood Donor No.6_stimulated PBMCs_STAT4 ChIP-Seq |
| GSM945613 |
Combined immunoprecipitated DNA from Blood Donor No.1,2,3_non-stimulated PBMCs_Control IgG ChIP-Seq |
|
| Relations |
| BioProject |
PRJNA168219 |
| SRA |
SRP013611 |
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