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| Status |
Public on Mar 27, 2013 |
| Title |
Gene expression signatures in miR-29c overexpressed gastric cancer cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To explain the mechanism that miR-29c affects the cell proliferation, we attempted to identify the miR-29c target genes in gastric carcinoma. The expression profiles in MKN45, MKN7 and MKN74 cells transfected with miR-29c oligo or Negative control oligo were obtained from microarray analysis. Then, the genes differentially expressed (Fold change >= 2.0) in miR-29c-transfected cells compared with negative control-transfected ones were identified in each cell lines, respectively. The differentially expressed genes shared among 3 cell lines were identified as the candidates for miR-29c targets.
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| Overall design |
Human gastric cancer cell lines, MKN45, MKN74 and MKN7 were transfected with miR-29c oligo or negative control oligo (n=2) (Ambion). At 24h after, total RNA was extracted and microarray analysis was performed. The genes with common expression changes among three cell lines miR-29c-transfected were identified as the candidates for miR-29c targets.
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| Contributor(s) |
Tsukamoto Y, Nakada C |
| Citation(s) |
23442884 |
| Submission date |
Jun 07, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Yoshiyuki Tsukamoto |
| E-mail(s) |
tuka@oita-u.ac.jp
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| Organization name |
Oita university, Faculty of Medicine
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| Department |
Molecular Pathology
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| Street address |
hasamacho idaigaoka 1-1
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| City |
Yufu-city |
| State/province |
Oita |
| ZIP/Postal code |
879-5593 |
| Country |
Japan |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (12)
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| GSM945750 |
MKN45, miR-29c, replicate 2 |
| GSM945751 |
MKN74, Negative control, replicate 1 |
| GSM945752 |
MKN74, Negative control, replicate 2 |
| GSM945753 |
MKN74, miR-29c, replicate 1 |
| GSM945754 |
MKN74, miR-29c, replicate 2 |
| GSM945755 |
MKN7, Negative control, replicate 1 |
| GSM945756 |
MKN7, Negative control, replicate 2 |
| GSM945757 |
MKN7, miR-29c, replicate 1 |
| GSM945758 |
MKN7, miR-29c, replicate 2 |
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| Relations |
| BioProject |
PRJNA168160 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38581_RAW.tar |
30.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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