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| Status |
Public on Dec 01, 2017 |
| Title |
Transcriptome-wide gene expression of the heart of neonatal rats with polygenic cardiac hypetrophy |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Identification of genes differentially expressed between the neonatal heart of a genetic rat model of cardiac hypertrophy (the Hypertrophic Heart Rat, HHR) and the control (the Normal Heart Rat, NHR) using Affymetrix GeneChip® Rat Gene 1.0 ST Arrays.
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| Overall design |
Neonatal (day 2) HHR and age-matched NHR were killed with an overdose of pentobarbitone (Lethobarb). The heart was immediately removed, and the ventricles were dissected from the atriums. The tissues were first preserved in liquid nitrogen and later transferred to a –80C freezer. Microarrays (one per sample, with no pooling) were performed on 8 neonatal HHR (4 male, 4 female) and 8 neonatal NHR (4 male, 4 female). After extraction of RNA, cRNA was prepared and arrays performed using Affymetrix GeneChip® Rat Gene 1.0 ST Arrays performed at the Ramaciotti Gene Function Analysis facility, University of New South Wales in Sydney, Australia.
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| Contributor(s) |
Marques FZ, Quarrell S, Chevalier C, Jayaswal V, Curl C, Yang JY, Lewandowski P, Delbridge L, Harrap S, Charchar FJ |
| Citation(s) |
28615213 |
| Submission date |
Jun 08, 2012 |
| Last update date |
Jun 19, 2019 |
| Contact name |
Francine Marques |
| E-mail(s) |
Francine.marques@bakeridi.edu.au
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| Phone |
61385321916
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| Organization name |
Baker IDI Heart and Diabetes
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| Street address |
Level 3, 75 Commercial Rd
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| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3004 |
| Country |
Australia |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA168209 |
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