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| Status |
Public on Nov 27, 2013 |
| Title |
Expression profiling of isolated dendritic arborization (da) neurons of Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Paper abstract: The transcription factors Abrupt (Ab) and Knot (Kn) act as selectors of distinct dendritic arbor morphologies in two classes of Drosophila sensory neurons, termed class I and class IV, respectively. We performed binding-site mapping and transcriptional profiling of isolated these neurons. Their profiles were similarly enriched in cell-type-specific enhancers of genes implicated in neural development. We identified a total of 429 target genes, of which 56 were common to Ab and Kn; these targets included genes necessary to shape dendritic arbors in either or both of the two sensory subtypes. Furthermore, a common target gene, encoding the cell adhesion molecule Ten-m, was expressed more strongly in class I than IV, and this differential was critical to the class-selective directional control of dendritic branch sprouting or extension. Our analyses illustrate how differentiating neurons employ distinct and shared repertoires of gene expression to produce class-selective morphological traits.
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| Overall design |
da neurons were isolated by magnetic bead sorting from larvae of the following three genotypes and obtained genome-wide expression profiles: larvae expressing a cell-surface marker mCD8:GFP alone (Control), and larvae ectopically expressing Abrupt (Ab ME) or Knot (Kn ME) together with mCD8:GFP in all classes of da neurons. 3 biological replicates were collected.
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| Contributor(s) |
Hattori Y, Usui T, Satoh D, Moriyama S, Shimono K, Itoh T, Shirahige K, Uemura T |
| Citation(s) |
24290980 |
| Submission date |
Jun 12, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Yukako Hattori |
| E-mail(s) |
yhattori@lif.kyoto-u.ac.jp
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| Phone |
+81-75-753-9240
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| Organization name |
Kyoto University
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| Department |
Graduate School of Biostudies
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| Lab |
Tadashi Uemura's lab
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| Street address |
Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (9)
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| GSM947122 |
Control cells from larva, biological replicate 1 |
| GSM947123 |
Control cells from larva, biological replicate 2 |
| GSM947124 |
Control cells from larva, biological replicate 3 |
| GSM947125 |
Ab ME cells from larva, biological replicate 1 |
| GSM947126 |
Ab ME cells from larva, biological replicate 2 |
| GSM947127 |
Ab ME cells from larva, biological replicate 3 |
| GSM947128 |
Kn ME cells from larva, biological replicate 1 |
| GSM947129 |
Kn ME cells from larva, biological replicate 2 |
| GSM947130 |
Kn ME cells from larva, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA168357 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38660_RAW.tar |
13.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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