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| Status |
Public on Sep 07, 2012 |
| Title |
Distinct roles for IL-27 and IFN-g in the development of T-bet+ Treg required to limit infection-induced pathology |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
IL-27 is a potent antagonist of TH1-mediated inflammation, but the basis for this effect is not fully understood. Recent studies identified a population of T-bet+ CXCR3+ Treg that limit TH1-mediated immune pathology. The studies presented here demonstrate that IL-27-mediated STAT1 activation promotes Treg expression of T-bet and CXCR3. Infection with Toxoplasma gondii induced a similar Treg population that limits T cell responses and this population at mucosal sites is IL-27-dependent. Furthermore, transfer of Treg ameliorated the infection-induced CD4+ T cell-mediated pathology observed in IL-27p28-/- mice. Although IFN-γ promoted a similar population of cells in the periphery, it did not compensate for the absence of IL-27 at mucosal sites and microarray analysis revealed that Treg exposed to either cytokine have distinct transcriptional profiles. These findings suggest that IFN-γ and IL-27 have different roles in Treg biology but define IL-27 as a key cytokine that promotes the development of Treg specialized to control TH1 immunity.
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| Overall design |
Three conditions were analyzed across two timepoints. Inducible regulatory T cells (iTreg) were generated in vitro in the presence of IL-27, IFNg or under 'Neutral' conditions as a control. Samples were collected at 10 hours and 2 days during the culture period. Three biological replicates were used for each condition.
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| Contributor(s) |
Beiting DP, O'Hara A, Hunter CA |
| Citation(s) |
22981537 |
| Submission date |
Jun 13, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
daniel beiting |
| E-mail(s) |
beiting@vet.upenn.edu
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| Organization name |
University of Pennsylvania
|
| Department |
Pathobiology
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| Lab |
Beiting
|
| Street address |
380 S. University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA168438 |
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