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| Status |
Public on Dec 07, 2012 |
| Title |
Evaluation of gene expression between CD44+ and CD44- cells in colonospheres |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify genes that are differentially expressed between CD44+ and CD44- cells in colonospheres, we have employed whole genome microarray expression profiling as a discovery platform. Colonospheres from four colon cancer patients were sorted into CD44+ and CD44- cells using the FACS Aria II Cell Sorter, and total RNA extracted from the cells were labeled with Cy3 and used for microarray analyses with Agilent Whole Human Genome Oligo Microarrays.
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| Overall design |
Gene expression in fluorescence-activated cell-sorted CD44+ and CD44- cells derived from four colon cancer patients was measured.
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| Contributor(s) |
Ohata H |
| Citation(s) |
23002207 |
| Submission date |
Jun 13, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Hirokazu Ohata |
| Organization name |
National Cancer Center Research Institute
|
| Street address |
Tsukiji 5-1-1
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA168453 |
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