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Status |
Public on Aug 10, 2012 |
Title |
Mouse ooplasm confers context-specific reprogramming capacity |
Organism |
Mus musculus |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
Enucleated oocytes have the remarkable ability to reprogram somatic nuclei back to totipotency. Here we investigate genome-scale DNA methylation patterns after nuclear transfer and identify specific targets for DNA demethylation as well as NT specific limitations. We find that the ooplasm can confer similar demethylation patterns in both processes except at some repetitive element classes and that germ-line associated promoters are specifically targeted in NT.
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Overall design |
Comparison of DNA methylation patterns in murine fertilization and somatic cell nuclear transfer
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Contributor(s) |
Chan MM, Smith ZD, Egli D, Regev A, Meissner A |
Citation(s) |
22902786 |
Submission date |
Jun 14, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Michelle M Chan |
Organization name |
UCSF
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Lab |
Weissman Lab
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Street address |
1700 4th St.
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
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Platforms (1) |
GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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Samples (9)
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Relations |
Reanalysis of |
GSM856495 |
Reanalysis of |
GSM856496 |
Reanalysis of |
GSM856497 |
Reanalysis of |
GSM856498 |
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GSM856499 |
Reanalysis of |
GSM856500 |
Reanalysis of |
GSM856505 |
Reanalysis of |
GSM856506 |
Reanalysis of |
GSM856507 |
Reanalysis of |
GSM856512 |
Reanalysis of |
GSM856513 |
Reanalysis of |
GSM856514 |
Reanalysis of |
GSM856515 |
Reanalysis of |
GSM856516 |
Reanalysis of |
GSM869999 |
Reanalysis of |
GSM870000 |
Reanalysis of |
GSM870001 |
Reanalysis of |
GSM870002 |
Reanalysis of |
GSM870003 |
Reanalysis of |
GSM870007 |
Reanalysis of |
GSM870008 |
Reanalysis of |
GSM870009 |
BioProject |
PRJNA168490 |
SRA |
SRP013748 |