|
| Status |
Public on Jun 15, 2012 |
| Title |
Time series data of HCV (JC1) infection of Huh7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Huh7/5-2 cells (Binder et al., Hepatology 2007) were mock infected (DMEM) (time points 4 and 48 h) or infected with the chimeric HCV virus Jc1 (Pietschmann et al., PNAS 2006) (all time points).
Multiplicity of infection was 15 (TCID50). Cells were lysed after 4, 12, 24, 48 and 72 hours post infection and total cellular RNA was prepared.
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| |
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| Overall design |
Mock infected cells serve as controls for the infected samples. The whole experiment was repeated on independent days (2 biological replicates).
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| |
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| Contributor(s) |
Binder M, Bartenschlager R, Trippler M, Kandasamy K, Pichlmair A, Superti-Furga G |
| Citation(s) |
22810585 |
| Submission date |
Jun 14, 2012 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Kumaran Kandasamy |
| E-mail(s) |
itskkumaran@gmail.com
|
| Phone |
4314016070050
|
| Fax |
4314016070050
|
| URL |
http://www.cemm.at
|
| Organization name |
Center for Molecular Medicine
|
| Street address |
Lazarettgasse 14, AKH BT25.3
|
| City |
Vienna |
| State/province |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (14)
|
| GSM948651 |
Huh7/5-2 uninfected 4 h biological replicate 1 |
| GSM948652 |
Huh7/5-2 uninfected 4 h biological replicate 2 |
| GSM948653 |
Huh7/5-2 infected 4 h biological replicate 1 |
| GSM948654 |
Huh7/5-2 infected 4 h biological replicate 2 |
| GSM948655 |
Huh7/5-2 infected 12 h biological replicate 1 |
| GSM948656 |
Huh7/5-2 infected 12 h biological replicate 2 |
| GSM948657 |
Huh7/5-2 infected 24 h biological replicate 1 |
| GSM948658 |
Huh7/5-2 infected 24 h biological replicate 2 |
| GSM948659 |
Huh7/5-2 uninfected 48 h biological replicate 1 |
| GSM948660 |
Huh7/5-2 uninfected 48 h biological replicate 2 |
| GSM948661 |
Huh7/5-2 infected 48 h biological replicate 1 |
| GSM948662 |
Huh7/5-2 infected 48 h biological replicate 2 |
| GSM948663 |
Huh7/5-2 infected 72 h biological replicate 1 |
| GSM948664 |
Huh7/5-2 infected 72 h biological replicate 2 |
|
| Relations |
| BioProject |
PRJNA168577 |
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