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Series GSE38736 Query DataSets for GSE38736
Status Public on Jun 30, 2013
Title Characterization of cholesterol loading effect on the gene expression of bone marrow derived macrophages of AKR and DBA/2 ApoE-deficient mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Strain effects on the response to cholesterol loading in bone marrow derived macrophages (BMM) are not well studied. The atherosclerosis susceptible strain, DBA/2 on the ApoE deficient background and the atherosclerosis resistant AKR strains show differences in cholesterol handling when loaded with AcLDL. To understand the molecular basis for these differences, BMM from both strains were loaded with acetylated low desnisty lipoprotein (AcLDL) and gene expression quantified. BMMs from AKR greatly upregulated transcripts involved in the lysosomal trafficking pathway. This experiment strongly suggest that upregualtion of the lysosomal pathway in response to AcLDL maybe protective against atherosclerosis. In addition we find that transcripts containing the sterol response element (SRE) motif had strong strain-loading interactions suggesting a strain response to SRE response.
Overall design 32 BMM cultures from AKR (n=16) and DBA/2 (n=16) mice of both genders (16 males and 16 females) were loaded with AcLDL (n=16) or control media (n=16) resulting in 4 biological replicates for each of the conditions, gender, loading, and strain.
Contributor(s) Berisha SZ, Hsu J, Smith JD
Citation(s) 23705026
Submission date Jun 14, 2012
Last update date Aug 13, 2019
Contact name Jeff Hsu
Phone 2162443723
Organization name Cleveland Clinic Lerner Research Institute
Department Cellular and Molecular Medicine
Lab Smith
Street address 9500 Euclid Ave
City Cleveland
State/province OH
ZIP/Postal code 44195
Country USA
Platforms (2)
GPL6103 Illumina mouseRef-8 v1.1 expression beadchip
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (32)
GSM948808 female_BMM_AKR_unloaded_1
GSM948809 female_BMM_AKR_unloaded_2
GSM948810 female_BMM_DBA_unloaded_1
BioProject PRJNA168590

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Supplementary file Size Download File type/resource
GSE38736_RAW.tar 6.5 Mb (http)(custom) TAR
Raw data included within Sample table
Processed data included within Sample table

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