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Series GSE38747 Query DataSets for GSE38747
Status Public on Jan 07, 2014
Title Regulatory role of multinucleated giant cells derived from placental CD14+ macrophages: Pathophysiological implications
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The role of placental macrophages is largely ignored in the success of pregnancy. We found that CD14+ macrophages purified from at-term placentas spontaneously matured into multinucleated giant cells (MGCs). MGCs lost the expression of CD14 and co-inhibitory molecules, such as Programmed cell Death-Ligands 1 and 2. Although MGCs kept phagocytosis and property to produce ROS, their inflammatory potential measured by TNF/IL-10 imbalance and activation of p38 MAPK and NF-κB was blunted without being associated with M2 phenotype. The investigation of gene expression revealed the enrichment with categories related to inflammation, apoptosis and canonical functions of macrophages in MGCs. Whereas most of the genes associated with inflammation and immune responses were down-modulated, those such as VEGFC or associated with matrix remodeling were specifically up-regulated in MGCs. Importantly, we found that patients with preeclampsia or chorioamnionitis, two inflammatory and infectious pathologies, respectively, that affect placentas, were unable to generate MGCs. Taken together, these results suggest that MGCs represent a new way to regulate the inflammatory and cytocidal activity of placental macrophages in a context that imposes contradictory constraints, such as semi-allograft acceptance and defense against aggression. The dysregulation of MGC formation may be associated with placental inflammatory and infectious pathologies.
Overall design Placental macrophages CD14+ were cultured for 9 days to form multinucleated giant cells (MGC). The transcriptome of MGCs was compared to the transcriptome of placental macrophages (CD14+)
Contributor(s) Ben Amara A, Gorvel L, Verollet C, Derain-Court J, Mehaj V, Barry AO, Textoris J, Bretelle F, Ghigo E, Capo C, Maridonneau-Parini I, Mège J
Citation(s) 24163411
Submission date Jun 15, 2012
Last update date Jan 23, 2019
Contact name Julien Textoris
Phone +33 472 119 546
Organization name bioMérieux
Department Medical Diagnostic Discovery Department (MD3)
Lab Joint Research Unit - bioMérieux / HCL
Street address Hôpital Edouard herriot - Pavillon P; 5 place d'Arsonval
City Lyon
ZIP/Postal code 69437
Country France
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (12)
GSM948986 MGC_Placenta1_rep1
GSM948987 CD14_Placenta2_rep1
GSM948988 MGC_Placenta2_rep1
BioProject PRJNA168560

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38747_RAW.tar 109.3 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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