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Series GSE38764 Query DataSets for GSE38764
Status Public on Aug 21, 2012
Title Transcriptome analysis of Drosophila neural stem cells reveals a transcriptional network for self-renewal.
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Drosophila neuroblasts have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type specific gene expression data. Here, we describe a methodology to isolate large numbers of pure neuroblasts and differentiating neurons that retain both cell cycle and lineage characteristics. We determine transcriptional profiles by mRNA sequencing and identify 28 predicted neuroblast specific transcription factors, which can be arranged in a network containing hubs for Notch signaling, growth control and chromatin regulation. Overexpression and RNAi for these factors identify Klumpfuss as a regulator of self-renewal. We show that loss of Klu function causes premature differentiation while overexpression results in the formation of transplantable brain tumors. Our data represent a valuable resource for Drosophila developmental neurobiology and we describes methodology that can be applied to other invertebrate stem cell lineages as well.
 
Overall design comparison of transcriptomes of Drosophila melanogaster larval neuroblasts and their differentiated daughter cells (neurons)
 
Contributor(s) Harzer H, Berger C, Knoblich JA
Citation(s) 22884370
Submission date Jun 15, 2012
Last update date May 15, 2019
Contact name Thomas Rainer Burkard
E-mail(s) thomas.burkard@imba.oeaw.ac.at
Organization name IMBA
Street address Dr. Bohrgasse 7
City Vienna
ZIP/Postal code 1030
Country Austria
 
Platforms (2)
GPL9061 Illumina Genome Analyzer II (Drosophila melanogaster)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (8)
GSM949245 wild type neuroblast 1
GSM949246 wild type neuroblast 2 (tech. Repl. 1)
GSM949247 wild type neuroblast 2 (tech. Repl. 2)
Relations
BioProject PRJNA168567
SRA SRP013767

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38764_DESeq.nbVsneuron-orig-FDR0.01.txt.gz 220.3 Kb (ftp)(http) TXT
GSE38764_RAW.tar 3.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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