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Status |
Public on Dec 31, 2014 |
Title |
Whole genome transcription profile of activated pre-B cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
To search for rapid changes in gene expression following pre-BCR activation, we performed DNA microarray analysis of activated pre-B cells with and without anti-m heavy chain treatment for 3 hour.The expression of only small subset of genes differed significantly.
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Overall design |
The mouse bone marrow derived pre-B cells were grown with IL-7 on limiting amounts of S17 stromal cells and activated by incubation with goat F(ab’)2 anti-mouse μ heavy chain (Southern Biotechnologies) at 5 μg/ml for 3 hour.
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Contributor(s) |
Jiyoti V, Thomas G |
Citation missing |
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Submission date |
Jun 18, 2012 |
Last update date |
Jan 16, 2019 |
Contact name |
Thomas Grundström |
E-mail(s) |
thomas.grundstrom@molbiol.umu.se
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Phone |
+46 90 785 2531
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Organization name |
Umeå University
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Department |
Department of Molecular Biology
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Lab |
TGN Lab
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Street address |
Analysvägen 1
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City |
Umeå |
ZIP/Postal code |
901 87 |
Country |
Sweden |
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Platforms (1) |
GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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Samples (4)
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Relations |
BioProject |
PRJNA168639 |