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| Status |
Public on Jan 24, 2013 |
| Title |
Gene expression analyses in IRF8-induced monocyte differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To understand the differentiation program in monocyte/macrophage differentiation, we performed ChIP-seq for IRF8 and H3K4me1 together with gene expression profiling during IRF8-induced monocyte differentiation. Both promoter-proximal and -distal binding of IRF8 associated with induction of the genes especially those related to monocytes/macrophages and immunity. DNA motif analysis for cis-regulatory elements of indirect IRF8 target genes predicted KLF4, essential for Ly6C+ monocyte development, to be a downstream transcription factor regulating the indirect target gene expression. Introduction of KLF4 into an Irf8-/- myeloid progenitor cell line induced a subset of IRF8 target genes and partially induced monocyte/macrophage differentiation. Together, this study revealed the genome-wide behavior of IRF8 and the IRF8-KLF4 axis during monocyte differentiation.
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| Overall design |
Gene expressions in monocyte-like cells differentiated by IRF8 or KLF4 were measured at day 4 after retroviral transductions to myeloid progenitor cell line, Tot2. Two independent experiments were performed.
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| Contributor(s) |
Nishiyama A, Tamura T |
| Citation(s) |
23319570 |
| Submission date |
Jun 19, 2012 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Tomohiko Tamura |
| E-mail(s) |
tamurat@yokohama-cu.ac.jp
|
| Organization name |
Yokohama City University
|
| Department |
Department of Immunology
|
| Street address |
3-9 Fukuura, Kanazawa-ku
|
| City |
Yokohama |
| ZIP/Postal code |
236-0004 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE38825 |
IRF8-induced monocyte differentiation |
|
| Relations |
| BioProject |
PRJNA168968 |
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