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| Status |
Public on Jun 21, 2012 |
| Title |
miR-200c is Aberrantly Expressed in Leiomyomas in Ethnic-Dependent Manner and Targets ZEBs, VEGFA, TIMP2 and FBLN5 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
mircoRNA-200c (miR-200c) through repression of specific target genes has been associated with cellular transition, tumorigenesis and tissue fibrosis. We explored the expression and functional aspects of miR-200c in genesis of leiomyomas, benign uterine tumors with fibrotic characteristic. Gain-of function of miR-200c in isolated leiomyoma and myometrial smooth muscle cells (LSMC and MSMC) and leiomyosarcoma cell line (SKLM-S1) repressed ZEB1/ZEB2 mRNAs and proteins, with concurrent increase in E-cadherin (CDH1) and reduction in vimentin expression, phenotypic alteration and inhibition of MSMC and LSMC proliferation. We further validated TIMP2, FBLN5 and VEGFA as direct targets of miR-200c through interaction with their respective 3’UTRs, and other genes as determined by microarray analysis.
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| Overall design |
Total RNA isolated from MSMC and LSMC following gain-of function of miR-200c was subjected to gene expression profiling using HumanHT-12 v4 Expression BeadChip (Illumina, Inc. San Diego, CA) consists of 47,000 oligonucleotide probe sets representing 28,688 transcripts.
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| |
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| Contributor(s) |
Chuang T, Panda H, Luo X, Chegini N |
| Citation(s) |
22685266 |
| Submission date |
Jun 19, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Tsai-Der Chuang |
| E-mail(s) |
chuangtsaide@gmail.com
|
| Phone |
310-222-1897
|
| Organization name |
LA Biomed at UCLA medical center
|
| Department |
OB-GYN
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| Lab |
Omid Khorram
|
| Street address |
1124 WEST CARSON STREET
|
| City |
Torrance |
| State/province |
California |
| ZIP/Postal code |
90502 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA169086 |
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