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| Status |
Public on Jun 21, 2012 |
| Title |
Conditional keratinocyte-specific ADAM17 knockout in mice alters skin homeostasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This study investigates the role of ADAM17 (a disintegrin and metalloproteinase 17) in skin homeostasis. Here, we show that mice lacking ADAM17 in keratinocytes have a normal epidermal barrier and skin architecture at birth, but develop pronounced defects in epidermal barrier integrity soon after birth and chronic dermatitis as adults. The dysregulated expression of epidermal differentiation proteins becomes evident 2 days after birth, followed by transepidermal water loss and inflammatory immune cell infiltration. Our results identify a previously unappreciated critical role of the ADAM17/EGFR signaling axis in maintaining the homeostasis of the postnatal epidermal barrier.
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| Overall design |
The genome-wide effects of ADAM17 deficiency were analyzed using Agilent Whole Mouse Genome microarrays. Conditional keratinocyte-specific ADAM17 knockout mice were generated by crossing Adam17flox/flox mice with keratin-14-Cre (Krt14-Cre) transgenic mice. Adam17flox/+Krt14-Cre mice were mated with Adam17flox/flox mice to generate pups of Adam17flox/flox Krt14-Cre positive (cKO) and Krt14-Cre negative (wild-type) control littermates. The genetic background was a mix of 129Sv and C57BL/6. As material, back skin tissue biopsies (postnatal day 10) from n = 2 wild-type skin and n = 2 ADAM17 epidermal KO skin (matched WT-cKO pairs from two different litters) were used in this study.
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| Contributor(s) |
Franzke C, Kurz T, Blobel C |
| Citation(s) |
22565824 |
| Submission date |
Jun 20, 2012 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Claus-Werner Franzke |
| E-mail(s) |
claus-werner.franzke@uniklinik-freiburg.de
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| Phone |
+49 761 27067850
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| Organization name |
University of Freiburg
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| Department |
Dermatology
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| Lab |
Molecular Dermatology I
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| Street address |
Hauptstr. 7
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| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA169088 |
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