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Series GSE38869 Query DataSets for GSE38869
Status Public on Jun 30, 2013
Title Cardiomyocyte MR dependent DOC/salt-mediated gene regulation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Genome wide gene expression analysis of mRNA siolated from whole heart tissue from wild type and cardiomyocyte selective MR null mice.
The role of mineralocorticoid receptors (MR) in specific cell types of the myocardium in cardiac remodeling remains unknown. We investigated MR in cardiomyocytes in DOC/salt-induced cardiac pathology. Cardiomyocyte MR-null mice (CM-MRKO) and control mice (WT) were given DOC/salt and cardiac responses were examined at 8 days and 8 weeks. Cardiac function in untreated mice wild type and CM-MRKO mice was determined by Langendorf and showed no differences. At 8 days CM-MRKO showed equivalent monocyte/macrophage recruitment to wild type mice in response to DOC/salt treatment. Profibrotic markers were significantly reduced in CM-MRKO hearts at base line and in response to DOC/salt. In contrast, at 8 weeks CM-MRKO mice showed no DOC/salt-induced increase in inflammatory cell infiltrate, fibrosis or systolic blood pressure (SBP). Similarly, DOC/salt-mediated increases in proinflammatory and profibrotic gene expression were not detected in CM-MRKO mice. Although mRNA levels for fibronectin and collagen III were similar for each genotype, this was not translated into protein expression. Interestingly, untreated CM-MRKO mice showed increased mRNA and protein for decorin and a further increase with DOC/salt. Together, these data suggest a direct role for cardiomyocyte MR in DOC/salt-induced tissue remodelling and SBP regulation. Moreover, a specific profibrotic pathway is dysregulated in CM-MRKO mice, suggesting a potential mechanism for the cardioprotective effects of selective MR deletion in cardiomyocytes.
 
Overall design Pool mRNA (equal amounts) from whole heart from 8 animals per group.
 
Contributor(s) Young M
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Submission date Jun 21, 2012
Last update date Jun 14, 2018
Contact name Morag J Young
Organization name Prince Henry's Institute of Medical Research
Lab Cardiovascular Endocrinology
Street address Level 4 Block E 246 Clayton Rd
City Clayton
State/province Victoria
ZIP/Postal code 3168
Country Australia
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM951088 heart-vehicle-pooled WT
GSM951089 heart-DOC/salt-pooled WT
GSM951090 heart-vehicle-pooled CM MRKO
Relations
BioProject PRJNA169083

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38869_RAW.tar 3.1 Mb (http)(custom) TAR
GSE38869_non_normalized.txt.gz 302.7 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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