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Series GSE38872 Query DataSets for GSE38872
Status Public on Aug 15, 2013
Title Liver gene expression profile _ wild type_ CYP7A1 transgenic
Organism Mus musculus
Experiment type Expression profiling by array
Summary Bile acids are not only physiological detergents facilitating nutrient absorption, but also signaling molecules regulating metabolic homeostasis. We reported recently that transgenic expression of CYP7A1 in mice stimulated bile acid synthesis and prevented Western diet-induced obesity, insulin resistance and hepatic steatosis. The aim of this experiment is to determine the impact of induction of hepatic bile acid synthesis on liver metabolism by determining hepatic gene expression profile in CYP7A1 transgenic mice. CYP7A1 transgenic mice and wild type control mice were fed either standard chow diet or high fat high cholesterol Western diet for 4 month. Hepatic gene expressions were measured by microarray analysis. Our results indicate that hepatic bile acid synthesis is closely linked to cholesterogenesis and lipogenesis, and maintaining bile acid homeostasis is improtant in hepatic metabolic homeostasis.
 
Overall design Male aged matched (~ 12-14 weeks) CYP7A1 transgenic mice and their wild type control littermates were fed a standard chow diet or a high fat (42%) high cholesterol (0.2%) diet (Harlan Teklad #88137) for 4 month Four groups (4 mice/group) are included in the experiments:
Group 1: WT _ Chow
Group 2: CYP7A1-tg + chow
Group 3: WT + Western diet
Group 4: CYP7A1-tg _ Western diet

Total liver mRNA was isolated with a RNeasy kit (Qiagen) and used for microarray analysis.
 
Contributor(s) Gopalan B, Boehme S, Cheng J, Gopalan B, Na J, Chiang J
Citation(s) 23536474
Submission date Jun 22, 2012
Last update date Jun 14, 2018
Contact name John Chiang
E-mail(s) Jchiang@neomed.edu
Organization name Northeast Ohio Medical University
Department Biochemistry and Molecular Pathology
Street address 4209 State Route 44
City Rootstown
State/province OH
ZIP/Postal code 44272
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM946456 WT-Chow-Liver-1
GSM946457 WT-Chow-Liver-2
GSM946458 WT-Chow-Liver-3
Relations
BioProject PRJNA169150

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38872_RAW.tar 6.2 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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