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Status |
Public on Jun 23, 2012 |
Title |
A novel 3-D organotypic culture model by co-culturing alpha-SMA positive CAF and cholangiocarcinoma cells in a collagen matrix |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
The increased α-smooth muscle-actin positive cancer-associated fibroblastic cells (CAF) in the desmoplastic stroma may relate to a more aggressive cancer and worse survival outcomes for intrahepatic cholangiocarcinoma (ICC) patients We developed a novel 3-D organotypic culture model by co-culturing α-SMA positive CAF and cholangiocarcinoma cells in a collagen matrix.
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Overall design |
Cholangiocarcinoma cell lines were established by isolating α-SMA positive cancer-associated fibroblastic cells (CAF) (BDEsp-TDFE4) and cholangiocarcinoma cells (BDEsp-TDEH10) from tumors arising from bile duct inoculation of spontaneously-transformed low grade malignant rat BDE1 cholangiocytes (BDEsp cells). These tumor-derived cells lines were then grown in a rat tail type I collagen gel matrix, alone or in co-culture, and their gene expression profile were compared.
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Contributor(s) |
Sirica A, Dumur CI, Campbell D, Lamour N |
Citation(s) |
22568523, 28315313 |
Submission date |
Jun 22, 2012 |
Last update date |
Jul 31, 2017 |
Contact name |
Catherine Dumur |
Organization name |
Sonic Healthcare USA
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Department |
Anatomic Pathology
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Lab |
Bernhardt
|
Street address |
5008 Mustang Rd
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City |
Jacksonville |
State/province |
FL |
ZIP/Postal code |
32216 |
Country |
USA |
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Platforms (1) |
GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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Samples (13)
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GSM951538 |
Tumor arising from BDEsp cells rep1 |
GSM951539 |
Tumor arising from BDEsp cells rep2 |
GSM951540 |
Tumor arising from BDEsp cells rep3 |
GSM951541 |
Tumor arising from BDEsp cells rep4 |
GSM951542 |
BDEsp Tumor-Derived Epithelial cells rep1 |
GSM951543 |
BDEsp Tumor-Derived Epithelial cells rep2 |
GSM951544 |
BDEsp Tumor-Derived Fibroblastic cells rep1 |
GSM951545 |
BDEsp Tumor-Derived Fibroblastic cells rep2 |
GSM951546 |
BDEsp Tumor-Derived Fibroblastic cells rep3 |
GSM951547 |
BDEsp Tumor-Derived Fibroblastic cells rep4 |
GSM951548 |
BDEsp Tumor-Derived Epithelial cells rep3 |
GSM951549 |
BDEsp Tumor-Derived Epithelial cells in Co-Culture with BDEsp Tumor-Derived Fibroblastic cells |
GSM951550 |
BDEsp Tumor-Derived Fibroblastic cells in Co-Culture with BDEsp Tumor-Derived Epithelial cells |
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Relations |
BioProject |
PRJNA169161 |