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Series GSE38889 Query DataSets for GSE38889
Status Public on Jun 23, 2012
Title A novel 3-D organotypic culture model by co-culturing alpha-SMA positive CAF and cholangiocarcinoma cells in a collagen matrix
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The increased α-smooth muscle-actin positive cancer-associated fibroblastic cells (CAF) in the desmoplastic stroma may relate to a more aggressive cancer and worse survival outcomes for intrahepatic cholangiocarcinoma (ICC) patients
We developed a novel 3-D organotypic culture model by co-culturing α-SMA positive CAF and cholangiocarcinoma cells in a collagen matrix.
 
Overall design Cholangiocarcinoma cell lines were established by isolating α-SMA positive cancer-associated fibroblastic cells (CAF) (BDEsp-TDFE4) and cholangiocarcinoma cells (BDEsp-TDEH10) from tumors arising from bile duct inoculation of spontaneously-transformed low grade malignant rat BDE1 cholangiocytes (BDEsp cells). These tumor-derived cells lines were then grown in a rat tail type I collagen gel matrix, alone or in co-culture, and their gene expression profile were compared.
 
Contributor(s) Sirica A, Dumur CI, Campbell D, Lamour N
Citation(s) 22568523, 28315313
Submission date Jun 22, 2012
Last update date Jul 31, 2017
Contact name Catherine Dumur
Organization name Sonic Healthcare USA
Department Anatomic Pathology
Lab Bernhardt
Street address 5008 Mustang Rd
City Jacksonville
State/province FL
ZIP/Postal code 32216
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (13)
GSM951538 Tumor arising from BDEsp cells rep1
GSM951539 Tumor arising from BDEsp cells rep2
GSM951540 Tumor arising from BDEsp cells rep3
Relations
BioProject PRJNA169161

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38889_RAW.tar 55.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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