GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE3889 Query DataSets for GSE3889
Status Public on Dec 23, 2005
Title Scd1 Knockout Mice on very low fat diet
Organism Mus musculus
Experiment type Expression profiling by array
Summary Stearoyl-CoA desaturase 1-deficient (SCD1-/-) mice have impaired monounsaturated fatty acid (MUFA) synthesis. When maintained on a very low-fat, high-carbohydrate (VLF-HC) diet, SCD1-/- mice develop severe hypercholesterolemia characterized by an increase in apolipoprotein B-containing lipoproteins and the appearance of lipoprotein-X. Additionally, high-density lipoprotein cholesterol is dramatically reduced in VLF-HC SCD1-/- mice. The concomitant presence of elevated plasma bile acids, bilirubin and aminotransferases in the VLF-HC SCD1-/- mouse are indicative of hepatic dysfunction. Supplementation of the VLF-HC diet with unsaturated fat (canola oil), but not saturated fat (coconut oil), prevents these plasma phenotypes. However, dietary oleate was not as effective as canola oil in reducing low-density lipoprotein cholesterol, signifying an additional role for dietary polyunsaturated fatty acid deficiency in the development of this phenotype. These results indicate that lack of SCD1 results in an increased requirement for dietary unsaturated fat to compensate for impaired MUFA synthesis and to prevent hypercholesterolemia and hepatic dysfunction.
Keywords: repeat (genotype and diet)
Overall design We used Affymetrix Mouse430v2.0 microarray chips to search for gene expression changes unique to the VLF-HC SCD1-/- group. These chips contain 45,101 probe sets representing over 39,000 transcripts and variants from over 34,000 mouse genes. We studied SCD1+/+ and SCD1-/- mice on chow or the VLF-HC diet, analyzing five individual livers from each subgroup for a total of twenty microarrays.
Contributor(s) Flowers MT, Oler A, Schueler KL, Gray-Keller MP, Richards OC, Lan H, Choi Y, Kuipers F, Groen A, Ntambi JM, Attie AD
Citation(s) 17005996
Submission date Dec 21, 2005
Last update date Feb 11, 2019
Contact name Alan D Attie
Phone 608-262-4705
Fax 608-263-9609
Organization name University of Wisconsin-Madison
Department Biochemistry
Lab Attie
Street address 433 Babcock Drive
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (20)
GSM88872 Scd1-/- on very low fat diet (TD03045 from Harlan Teklad) replicate 1
GSM88873 Scd1-/- on very low fat diet (TD03045 from Harlan Teklad) replicate 2
GSM88874 Scd1-/- on very low fat diet (TD03045 from Harlan Teklad) replicate 3
BioProject PRJNA94131

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3889_RAW.tar 69.8 Mb (http)(custom) TAR (of CEL)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap