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Series GSE3895

Query DataSets for GSE3895

Status

Public on Dec 24, 2005

Title

Coordinated regulation of gene expression by Brn3a in developing sensory ganglia

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Mice lacking the POU-domain transcription factor Brn3a exhibit marked defects in sensory axon growth and abnormal sensory apoptosis. We have determined the regulatory targets of Brn3a in the developing trigeminal ganglion using microarray analysis of Brn3a mutant mice. These results show that Brn3 mediates the coordinated expression of neurotransmitter systems, ion channels, structural components of axons and inter- and intracellular signaling systems. Loss of Brn3a also results in the ectopic expression of transcription factors normally detected in earlier developmental stages and in other areas of the nervous system. Target gene expression is normal in heterozygous mice, consistent with prior work showing that autoregulation by Brn3a results in gene dosage compensation. Detailed examination of the expression of several of these downstream genes reveals that the regulatory role of Brn3a in the trigeminal ganglion appears to be conserved in more posterior sensory ganglia but not in the CNS neurons that express this factor.
Keywords: Gene expression in the developing nervous system

Overall design

Microarrays used to compare the patterns of gene expression in the trigeminal ganglia of Brn3a knockout and wild-type mice. Embryonic day 13.5 (E13.5) was chosen because at this point in development mutant mice exhibit major defects in sensory axon growth, but have yet to undergo the period of extensive sensory neuron death associated with later stages.

Contributor(s)

Eng SR, Lanier J, Natalia F, Turner EE

Citation(s)

15253936

Submission date

Dec 22, 2005

Last update date

Feb 18, 2018

Contact name

Eric Turner

E-mail(s)

eturner@ucsd.edu