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Series GSE38962 Query DataSets for GSE38962
Status Public on Jun 28, 2012
Title Antisense oligonucleotides targeting nuclear RNA for in vivo correction of myotonic dystrophy
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study examined the effects of antisense oligonucleotides (ASOs) on the muscle transcriptome in a transgenic mouse model of myotonic dystrophy. Two ASOs were tested in HSALR transgenic mice. Both of the ASOs targeted mRNA from a human skeletal actin (hACTA1) transgene. This transgene contains an expanded CTG repeat in the 3’ untranslated region (UTR) (Mankodi et al “Myotonic dystrophy in transgenic mice expressing an expanded CUG repeat” Science 2000; 289:1769-72). ASO 445235 targeted the hACTA1 transcript in the 3’ UTR, downstream from the expanded repeat. ASO 190401 targeted the hACTA1 transcript in the coding region. The HSALR mice received 4 weeks of biweekly subcutaneous injections of vehicle (saline), ASO 190401, or ASO 445236 (n = 4 per group), at a dose of 25 mg/kg per injection. Wild-type mice of the same strain background received saline injections (n = 4). One week after the final injection, quadriceps muscle was harvested for RNA analysis.
 
Overall design Four conditions, four arrays per condition, to compare WT and HSALR trangenic mice without treatment (saline) and to examine the effect of two oligos (vs. saline) in the HSALR transgenic mice.
 
Contributor(s) Thornton CA
Citation(s) 22859208
Submission date Jun 27, 2012
Last update date Jun 14, 2018
Contact name Stephen Welle
E-mail(s) swelle@rochester.rr.com
Organization name University of Rochester
Street address 601 Elmwood Avenue
City Rochester
State/province NY
ZIP/Postal code 14642
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM952939 HSALR-ASO445236 5543266006_A
GSM952940 HSALR-saline 5543266006_B
GSM952941 HSALR-saline 5543266006_C
Relations
BioProject PRJNA169527

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38962_RAW.tar 3.1 Mb (http)(custom) TAR
GSE38962_non-normalized.txt.gz 1.7 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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