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Status |
Public on Apr 23, 2013 |
Title |
Choriodecidual Infection Downregulates Angiogenesis and Morphogenesis Pathways in Fetal Lungs from Macaca Nemestrina |
Platform organism |
Macaca mulatta |
Sample organism |
Macaca nemestrina |
Experiment type |
Expression profiling by array
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Summary |
Intrauterine exposure to amniotic fluid (AF) cytokines is thought to predispose to bronchopulmonary dysplasia (BPD). We evaluated the effects of GBS exposure on RNA expression in fetal lung tissue to determine early molecular pathways associated with fetal lung injury that may progress to BPD.
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Overall design |
Ten chronically catheterized pregnant monkeys (Macaca nemestrina) at 118-125 days gestation (term=172 days) received choriodecidual inoculation of either: 1) Group B Streptococcus (n=5) or 2) saline (n=5). Cesarean section and fetal necropsy was performed in the first week after GBS or saline inoculation regardless of labor. RNA was extracted from fetal lungs and profiled by microarray. Results were analyzed using single gene, Gene Set, and Ingenuity Pathway Analysis. Validation was by RT-PCR and immunohistochemistry.
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Contributor(s) |
Bammler TK, Beyer RP, McAdams RM, Vanderhoeven J, Farin FM, Gravett MG, Rubens CE, Adams-Waldorf KM |
Citation(s) |
23056493 |
Submission date |
Jun 29, 2012 |
Last update date |
Sep 05, 2017 |
Contact name |
James William MacDonald |
E-mail(s) |
jmacdon@uw.edu
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Organization name |
University of Washington
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Department |
Environmental and Occupational Health Sciences
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Street address |
4225 Roosevelt Way NE
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City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98105-6099 |
Country |
USA |
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Platforms (1) |
GPL3535 |
[Rhesus] Affymetrix Rhesus Macaque Genome Array |
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Samples (10)
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Relations |
BioProject |
PRJNA169645 |