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| Status |
Public on Jul 12, 2012 |
| Title |
Genome-wide binding sites of Hypoxia inducible factor 1 (HIF1) and histone modifications |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the high-throughput profilings of HIF1 and histone modifications in human umbilical vein endothelial cells (HUVEC). By obtaining over two billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of HUVEC under normoxia and hypoxia. We find that HIF1binds to not only to transcriptional starting sites but also enhancer regions and that HIF1 binding sites were overlapped with lysine 4 trimethylatio, monomethylation and lysine 27 acetylation . Finally, we show that chromatin state can change under hypoxia by using chromatin conformational capture assay. This study provides novel insights into the interaction between HIF1 and KDM3A and also the epigenetic regulation of HIF1.
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| Overall design |
Examination of HIF1 and 3 different histone modifications in HUVEC under 2 conditions.
Related gene expression data is provided in GSE35932.
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| Contributor(s) |
Mimura I, Kanki Y, Kohro T, Yamamoto S, Wada Y, Kodama T |
| Citation(s) |
22645302 |
| Submission date |
Jul 03, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Takahide Kohro |
| E-mail(s) |
kohro@lsbm.org
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| Phone |
81-3-5452-5237
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| Organization name |
LSBM, RCAST
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| Street address |
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| City |
4-6-1, Komaba, Meguroku |
| State/province |
Tokyo |
| ZIP/Postal code |
153-8904 |
| Country |
Japan |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA169935 |
| SRA |
SRP014007 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39089_HUVEC_HIF1_hypoxia.bed.gz |
68.1 Kb |
(ftp)(http) |
BED |
| GSE39089_RAW.tar |
278.7 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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