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Series GSE3915 Query DataSets for GSE3915
Status Public on Jul 01, 2006
Title PKCa -/- vs wt small intestine
Organism Mus musculus
Experiment type Expression profiling by array
Summary Members of the PKC family of serine / threonine kinases play key regulatory roles in numerous cellular processes including differentiation and proliferation. Of the eleven mammalian PKC isoforms known several have been implicated in tumor development and progression. However, in most cases isotype specificity is poorly defined and even contrary functions for a single PKC have been reported, mostly because appropriate molecular and genetic tools were missing to specifically assess the contribution of single PKC isoforms in vivo.
In this report we therefore used PKC genetic targeting to study the role of PKCa and PKCz in colorectal cancer. Both isoforms were found to be strongly down-regulated in intestinal tumors of ApcMin/+ mice. A deletion of PKCz did not affect tumorigenesis in this animal model. In contrast, PKCa deficient ApcMin/+ mice developed more aggressive tumors and died significantly earlier than their PKCa proficient littermates. Even without an additional Apc mutation PKCa knock out mice showed an elevated tendency to develop spontaneous intestinal tumors. Transcriptional profiling revealed a role for this kinase in regulating EGFR signaling and proposed a synergistic mechanism for EGFR / AP-1 and WNT / APC pathways in mediating intestinal tumor development.
Keywords: genetic modification
 
Overall design Adult male animals (PKCa -/- and congenic wt controls; 50-60 days old) were sacrificed by cervical dislocation. Small intestines were dissected and total RNA (~5µg per sample) was extracted using the QIAGEN RNeasy kit.
Sample processing (performed at the RZPD, Deutsches Ressourcenzentrum für Genomforschung GmbH, Heubnerweg 6, 14059 Berlin) included cRNA generation and labeling with biotin. cRNA samples were hybridized to the GeneChips and chips stained with a streptavidine-phycoerythrine conjugate,
washed and scanned. Hybridization images were analyzed using
GCOS™ 1.1.
 
Contributor(s) Oster H, Leitges M
Citation(s) 16849539
Submission date Dec 28, 2005
Last update date Jan 08, 2019
Contact name Henrik Oster
E-mail(s) henrik.oster@uni-luebeck.de
Organization name University of Lübeck
Department CBBM
Street address Marie Curie Street
City Lübeck
ZIP/Postal code 23562
Country Germany
 
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (4)
GSM89632 wild-type intestine Chip 4
GSM89633 wild-type intestine Chip 5
GSM89634 PKCa -/- intestine Chip 8
Relations
BioProject PRJNA94171

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Supplementary data files not provided

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