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| Status |
Public on Aug 29, 2018 |
| Title |
Genes regulated/modulated by jagged1 in endothelial cells during inflammation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Proinflammatory activation of endothelial cells leads to recruitment of leukocytes by upregulation of adhesion molecules and presentation of chemoattractants. In response to such activation there is also a strong shift in the endothelial expression of Notch ligands, with downregulation of Dll4 and a upregulation of JAG1. To assess whether Jagged1 would affect the endothelial activation profile, we suppressed JAG1 expression during IL-1β-induced activation by means of siRNA and performed a genome-wide transcriptome analysis. Our results show for the first time that Jagged1 modulates the transcription profile of activated endothelial cells and describe data that imply a role for Jagged1 in sharpening the inflammatory profile of the vasculature, giving it an edge towards leukocyte recruitment. These findings imply that Jagged1 might be a potential therapeutic target to attenuate inflammation and reduce tissue damage in inflammatory diseases.
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| Overall design |
Genome-wide analysis of total RNA obtained from confluent human umbilival vein endothelial cells (pool of 7 individual donors) transfected with JAG1 siRNA or scrambled siRNA, and further subjected to proinflammatory activation by IL-1β 24 hours, compared to unstimulated controls (3 biological replicates in each group) (see below for the four resulting sample groups) .
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| Contributor(s) |
Edelmann RJ, Sundlisæter E, Haraldsen G |
| Citation(s) |
29449332 |
| Submission date |
Jul 09, 2012 |
| Last update date |
Aug 29, 2018 |
| Contact name |
Guttorm Haraldsen |
| E-mail(s) |
guttorm.haraldsen@rr-research.no
|
| Organization name |
Oslo University Hospital
|
| Department |
Department for Pathology
|
| Lab |
LIIPAT
|
| Street address |
Sognsvannsveien 20
|
| City |
OSLO |
| State/province |
OSLO |
| ZIP/Postal code |
0027 |
| Country |
Norway |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (12)
|
| GSM957616 |
HUVEC scrambled siRNA + IL-1β 24 hours rep1 |
| GSM957617 |
HUVEC scrambled siRNA unstimulated rep1 |
| GSM957618 |
HUVEC JAG1 siRNA + IL-1β 24 hours rep1 |
| GSM957619 |
HUVEC JAG1 siRNA unstimulated rep1 |
| GSM957620 |
HUVEC scrambled siRNA + IL-1β 24 hours rep2 |
| GSM957621 |
HUVEC scrambled siRNA unstimulated rep2 |
| GSM957622 |
HUVEC JAG1 siRNA + IL-1β 24 hours rep2 |
| GSM957623 |
HUVEC JAG1 siRNA unstimulated rep2 |
| GSM957624 |
HUVEC scrambled siRNA + IL-1β 24 hours rep3 |
| GSM957625 |
HUVEC scrambled siRNA unstimulated rep3 |
| GSM957626 |
HUVEC JAG1 siRNA + IL-1β 24 hours rep3 |
| GSM957627 |
HUVEC JAG1 siRNA unstimulated rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE85987 |
Inhibition of endothelial Notch signaling attenuates inflammation |
|
| Relations |
| BioProject |
PRJNA170167 |
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