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Series GSE3921 Query DataSets for GSE3921
Status Public on Dec 29, 2005
Title Embryonic stem cells and pluripotent germ cell tumors
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Remarkably little is known about the transcriptional profiles of human embryonic stem (ES) cells or the molecular mechanisms that underlie their pluripotency. To identify commonalties among the transcriptional profiles of different human pluripotent cells and to search for clues into the genesis of human germ cell tumors, we compared the expression profiles of human ES cell lines, human germ cell tumor cell lines and tumor samples, somatic cell lines, and testicular tissue samples by using cDNA microarray analysis. Hierarchical cluster analysis of gene expression profiles showed that the five independent human ES cell lines clustered tightly together, reflecting highly similar expression profiles. The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples. We identified 895 genes that were expressed at significantly greater levels in human ES and embryonal carcinoma cell lines than in control samples. These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype.
An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
Keywords: all_pairs
 
Overall design Using regression correlation
 
Contributor(s) Chen X, Chang H, Jones S, Chi J
Citation(s) 14595015
Submission date Dec 28, 2005
Last update date Jul 29, 2013
Organization Stanford Microarray Database (SMD)
E-mail(s) array@genome.stanford.edu
Phone 650-498-6012
URL http://genome-www5.stanford.edu/
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (8)
GPL1283 SHBR
GPL2932 SHAX
GPL3038 SHAW
Samples (74)
GSM81469 GC (SNU 16 cells)
GSM81470 GC (HUVEC1)
GSM81471 GC (HUVEC2)
Relations
BioProject PRJNA94175

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Supplementary data files not provided

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