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Status |
Public on Dec 29, 2005 |
Title |
Embryonic stem cells and pluripotent germ cell tumors |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Remarkably little is known about the transcriptional profiles of human embryonic stem (ES) cells or the molecular mechanisms that underlie their pluripotency. To identify commonalties among the transcriptional profiles of different human pluripotent cells and to search for clues into the genesis of human germ cell tumors, we compared the expression profiles of human ES cell lines, human germ cell tumor cell lines and tumor samples, somatic cell lines, and testicular tissue samples by using cDNA microarray analysis. Hierarchical cluster analysis of gene expression profiles showed that the five independent human ES cell lines clustered tightly together, reflecting highly similar expression profiles. The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples. We identified 895 genes that were expressed at significantly greater levels in human ES and embryonal carcinoma cell lines than in control samples. These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Keywords: all_pairs
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Overall design |
Using regression correlation
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Contributor(s) |
Chen X, Chang H, Jones S, Chi J |
Citation(s) |
14595015 |
Submission date |
Dec 28, 2005 |
Last update date |
Jul 29, 2013 |
Organization |
Stanford Microarray Database (SMD) |
E-mail(s) |
array@genome.stanford.edu
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Phone |
650-498-6012
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URL |
http://genome-www5.stanford.edu/
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Department |
Stanford University, School of Medicine
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Street address |
300 Pasteur Drive
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City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
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Platforms (8)
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Samples (74)
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Relations |
BioProject |
PRJNA94175 |