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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 01, 2014 |
| Title |
Siah2-dependent gene expression profile |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling was performed to identify Siah2-dependent changes in cells subjected to ER stress, hypoxia, and combined glucose/oxygen deprivation. To establish the magnitude of the Siah2 effect on the ER stress response, we have compared gene expression profiles of WT and Siah1a-/-::Siah2-/- mouse embryo fibroblasts (MEFs) that were subjected to glycosylation inhibitor tunicamycin (TM), thapsigargin (TG), glucose deprivation, or glucose/oxygen deprivation. Overall, this analysis confirmed changes associated with ER stress that had not previously been associated with Siah2 signaling, substantiating Siah2 as a key coordinator of ER stress through the ATF4 and sXbp1 pathways.
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| Overall design |
WT and Siah1a/Siah2 KO MEF cells were treated with TM or TG for 6 h, or subjected for 12 h to oxygen deprivation, glucose deprivation or a combination of oxygen and glucose deprivation, in duplicate.
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| Contributor(s) |
Scortegagna M, Li JL, Ronai ZA |
| Citation(s) |
24809345 |
| Submission date |
Jul 10, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Jian-Liang Li |
| Organization name |
NIEHS
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| Street address |
111 TW Alexander Dr
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA170320 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39244_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE39244_non-normalized.txt.gz |
6.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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