|
| Status |
Public on Jul 12, 2012 |
| Title |
Gene expression analysis during reprogramming with sequential infecion of Oct4/Klf4/cMyc/Sox2 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To further understand the mechanism of reprogramming, the mouse embryonic fibroblast cells were infected with Oct4, Klf4, c-Myc and Sox2 with special sequence. 1) Oct4 and Klf4 containing retrovirus was delivered on day 0. 2) Oct4, Klf4 and c-Myc were delivered on Day 1.5. 3) c-Myc and Sox-2 were deivered on Day 3. 4) Sox2 were delivered on Day 4.5. 5) Vitamin C containging medium were used from Day 6. The gene expression of Day 0 (MEF), Day 1.5, Day 3, Day 4.5 and Day 6 were analyzed with micro array to indentify the possible underlying mechanism. Especially the factors related to MET/EMT, cell cycle and epigenetic were focused.
|
| |
|
| Overall design |
MEF cells were subjected for reprogramming with special protoocl: 1) Oct4 and Klf4 containing retrovirus were delivered on day 0. 2) Oct4, Klf4 and c-Myc were delivered on Day 1.5. 3) c-Myc and Sox-2 were deivered on Day 3. 4) Sox2 were delivered on Day 4.5. 5) Vitamin C containging medium were used from Day 6. RNA on Day 0 (MEF), Day 1.5, Day 3, Day 4.5 and Day 6 were collected for analysis.
|
| |
|
| Contributor(s) |
Zheng H, Liu X |
| Citation(s) |
23708003 |
| Submission date |
Jul 11, 2012 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Hui Zheng |
| E-mail(s) |
zheng_hui@gibh.ac.cn
|
| Phone |
86-20-32015334
|
| Fax |
86-20-32015231
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health, Chinese Academic of Science
|
| Department |
Stem Cell
|
| Street address |
190 Kaiyuan Ave. Science City
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA170418 |
Less...
More...