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| Status |
Public on Dec 30, 2005 |
| Title |
Inventory of genes expressed in human control hippocampus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by SAGE
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| Summary |
Serial analysis of gene expression (SAGE) was used to get a global view of the gene profile in human hippocampus. A library were generated from control hippocampus, obtained by rapid autopsy.
Keywords: hippocampus human inventory genes
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| Overall design |
Control hippocampus (used to construct the SAGE control library) was obtained from a 48 years old man without history of seizures or other neurological diseases and no brain abnormalities at autopsy and histologically normal hippocampus. Autopsy was performed within 4 hours after death. Tissue was snap-frozen and stored at –80 0C until use. Total RNA was isolated from control hippocampus and hippocampal surgical specimens, using the Trizol method according to the manufacturer’s instructions (Invitrogen - Life Technologies, The Netherlands). Part of the anterior hippocampus of control (including sectors CA1- CA4 and dentate gyrus, DG) was used. Poly(A)+ RNA isolation, cDNA synthesis and all subsequent steps of the SAGE procedure were essentially performed as described (Velculescu et al., 1995) with minor modifications given previously (Michiels et al., 1999).
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| Contributor(s) |
Redeker S, Aronica E |
| Citation(s) |
16413123 |
| Submission date |
Dec 29, 2005 |
| Last update date |
Apr 18, 2012 |
| Contact name |
Aronica Eleonora |
| E-mail(s) |
e.aronica@amc.uva.nl
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| Phone |
0031-20-5662943
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| Fax |
0031`-20-6914738
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| Organization name |
AMC
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| Department |
Neuropathology
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| Street address |
Meibergdreef 9
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| City |
Amsterdam |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL4 |
SAGE:10:NlaIII:Homo sapiens |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA94183 |
| Supplementary data files not provided |
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