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Series GSE39379 Query DataSets for GSE39379
Status Public on Dec 31, 2012
Title Notch signaling/MyoD double deficient embryonic muscle progenitors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Skeletal muscle growth and regeneration rely on myogenic progenitor and satellite cells, the stem cells of postnatal muscle. Elimination of Notch signals during mouse development results in premature differentiation of myogenic progenitors and formation of very small muscle groups. Here we show that this drastic effect is rescued by mutation of the muscle differentiation factor MyoD. However, rescued myogenic progenitors do not assume a satellite cell position and contribute poorly to myofiber growth. The disrupted homing is due to a deficit in basal lamina assembly around emerging satellite cells and to their impaired adhesion to myofibers. On a molecular level, emerging satellite deregulate the expression of basal lamina components and adhesion molecules like integrin a7, collagen XVIIIa1, Megf10 and Mcam. We conclude that Notch signals control homing of satellite cells, stimulating them to contribute to their own microenvironment and to adhere to myofibers.
 
Overall design Gene expression analysis using total RNA from FACS-isolated Vcam-1+/CD31-/CD45-/Sca1- embryonic muscle progenitor cells from E17.5 back muscle tissue of MyoD-/-, Pax3cre/+;Rbpjflox/flox;MyoD-/- and Pax3cre/+;DnMamlflox/flox;MyoD-/- mice.
 
Contributor(s) Bröhl D, Vasyutina E, Czajkowski MT, Griger J, Rassek C, Rahn H, Purfürst B, Wende H, Birchmeier C
Citation(s) 22940113
Submission date Jul 16, 2012
Last update date Jun 14, 2018
Contact name Dominique Bröhl
Organization name Max-Delbrück-Center for Molecular Medicine
Department Research Program Function and Dysfunction of the Nervous System
Lab Developmental Biology/Signal Transduction Group
Street address Robert-Rössle-Str. 10
City Berlin
State/province Berlin
ZIP/Postal code 13092
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (47)
GSM967035 wild type rep1
GSM967036 wild type rep2
GSM967037 wild type rep3
Relations
BioProject PRJNA170741

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39379_RAW.tar 3.1 Mb (http)(custom) TAR
GSE39379_non-normalized.txt.gz 7.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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