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| Status |
Public on Jul 04, 2013 |
| Title |
Chromatin landscape of progenitor motor neurons [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Progenitor motor neurons can be generated with high-efficiency by differentiating ES cells in vitro in the presence of retinoic acid and hedgehog signalling. Here, we characterize the chromatin landscape associated with progenitor motor neurons (pMNs) in order to assess how histone modification domains shift during the differentiation process.
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| Overall design |
In this study, we characterize the genomic occupancy of H3K27me3, H3K4me3, H3K79me2 and Pol2 using ChIP-seq in progenitor motor neurons that have been differentiated in vitro from ES cells.
An appropriate whole-cell extract control experiment for these ChIP-seq experiments is also included.
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| Contributor(s) |
Mahony S, Mazzoni EO, Whyte WA, Young RA, Gifford DK, Wichterle H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 17, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Shaun Mahony |
| E-mail(s) |
mahony@psu.edu
|
| Phone |
814-865-3008
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| Organization name |
Penn State University
|
| Department |
Biochemistry & Molecular Biology
|
| Lab |
Shaun Mahony
|
| Street address |
404 South Frear Bldg
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| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (5)
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| GSM968714 |
H3K27me3 in progenitor motor neurons [ChIP-seq] |
| GSM968715 |
H3K4me3 in progenitor motor neurons [ChIP-seq] |
| GSM968716 |
H3K79me2 in progenitor motor neurons [ChIP-seq] |
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| Relations |
| BioProject |
PRJNA170869 |
| SRA |
SRP014445 |
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