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| Status |
Public on Dec 31, 2013 |
| Title |
An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells (Dazl knockdown) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Primordial germ cells (PGCs) are the embryonic precursors to egg and sperm. When removed from the embryonic gonad, PGCs can give rise to embryonic germ cell lines (EGs), pluripotent stem cells that display most of the characteristics of embryonic stem cells (ESCs) including the ability to form teratomas and to contribute to chimera formation. In mice, EG cells can be derived between E8.5 up to E12.5 of embryonic development, at which point the PGCs undergo sexual differentiation and in the male transition into unipotent gonocytes. Dazl, a germ cell-specific RNA-binding protein, is specifically expressed in developing PGCs and is required for proper germ cell development. Dazl knockout mice are infertile, but the molecular mechanisms underlying this phenotype are still unknown. Here we demonstrate that Dazl localizes in granular structures in mammalian PGCs but not in ESCs. We demonstrate Dazl plays a central role in a large mRNA/protein interactive network that includes members of Fragile-X family RNA-binding proteins. We demonstrate that Dazl and Fxr1 play a central role in these granules and directly regulate the translation of specific core pluripotency factors, including Sox2 and Suz12.
Global gene expression changes following Dazl knockdown in in vitro primordial germ cells.
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| Overall design |
In vitro primordial germ cells carrying control and Dazl knockdown shRNAs were generated from Oct4-GFP ES cells and isolated by FACS analysis. The global gene expression profiles were analyzed by Agilent Mouse Whole Genome 4X44K one-color microarrays.
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| Contributor(s) |
Chen HH, Bloch DB, Mientjes E, Munoz J, Chen X, Tramp C, Wu J, Yabuuchi A, Chou YF, Buecker C, Welling M, Krainer A, Wilemsen R, Geijsen N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 20, 2012 |
| Last update date |
May 10, 2018 |
| Contact name |
Niels Geijsen |
| Organization name |
Massachusetts General Hospital
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| Department |
Center for Regenerative Medicine
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| Street address |
185 Cambridge St. CPZN 4-4256
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE39547 |
An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells |
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| Relations |
| BioProject |
PRJNA171233 |
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