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| Status |
Public on Jul 25, 2012 |
| Title |
Effects of breaking up prolonged sitting on skeletal muscle gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Breaking up prolonged sitting has been shown to be beneficially associated with cardio-metabolic risk markers in both observational and intervention studies. We aimed to define the acute transcriptional events induced in skeletal muscle by breaks in sedentary time.
Overweight/obese adults participated in a randomized three-period, three-treatment cross-over trial in an acute setting (5 hours): seated position with no activity, seated with 2-minute bouts of light- or moderate-intensity treadmill walking every 20 minutes. Vastus lateralis biopsies were obtained in 8 of the 19 participants after each treatment and gene expression examined using microarrays validated with real-time qPCR.
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| |
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| Overall design |
Total RNA was extracted from vastus lateralis at the 3 different experimental condition
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| |
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| Contributor(s) |
Jowett JB, Latouche C |
| Citation(s) |
23271697 |
| Submission date |
Jul 23, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Celine Latouche |
| E-mail(s) |
celine.latouche@bakeridi.edu.au
|
| Phone |
0385321375
|
| Organization name |
Baker IDI Heart and Diabetes Institute
|
| Lab |
Metabolic and Vascular Physiology
|
| Street address |
75 Commercial Road
|
| City |
Melbourne |
| State/province |
VICTORIA |
| ZIP/Postal code |
3181 |
| Country |
Australia |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA171152 |
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