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Series GSE3962 Query DataSets for GSE3962
Status Public on Jun 30, 2006
Title Mouse oocyte and one-cell embryo polysomal mRNA
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptional activation in mammalian embryos occurs in a stepwise manner. In mice, it begins at the late one-cell stage, followed by a minor wave of activation at the early two-cell stage, and then the major genome activation (MGA) at the late two-cell stage. Cellular homeostasis, metabolism, cell cycle, and developmental events are orchestrated before MGA by time-dependent changes in the array of maternal transcripts being translated (i.e., the translatome). Despite the importance of maternal mRNA and its correct recruitment for development, neither the array of recruited mRNA nor the regulatory mechanisms operating have been well cheracterized. We present the first comprehensive analysis of changes in the maternal component of the zygotic translatome during the transition from oocyte to late one-cell stage embryo, revealing global transitions in the functional classes of translated maternal mRNAs, and apparent changes in the underlying cis-regulatory mechanisms.
Keywords: Time course
Overall design Batches of 160-500 MII oocytes or one-cell embryos. 4 independent replicates per sample kind. Lysed and processed for polysomal mRNA extraction. Two rounds of IVT.
Contributor(s) Latham KE, Potireddy S, Vassena R
Citation(s) 16860309
Submission date Jan 03, 2006
Last update date Feb 11, 2019
Contact name Keith E Latham
Organization name Temple University School of Medicine
Department The Fels Institute for Cancer Research and Molecular Biology
Street address 3307 N Broad St
City Philadelphia
State/province PA
ZIP/Postal code 19140
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM89969 MII oocyte polysomes sample 1
GSM89970 MII oocyte polysomes sample 2
GSM89971 MII oocytes polysomes sample 3
BioProject PRJNA94219

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