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Status |
Public on Sep 18, 2012 |
Title |
Identification of the hemogenic endothelial progenitor and its direct precursor in human pluripotent stem cell differentiation cultures |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Hemogenic endothelium (HE) is the source of HSCs in the developing embryo. In this study we have identified the hemogenic endothelial progenitors and their precursors originating from differentiated H1 cells on OP9 stromal cells.
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Overall design |
RNA-seq of hemogenic endothelial progenitors and their precursors originating from differentiated H1 cells on OP9 stromal cells.
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Contributor(s) |
Choi K, Vodyanik M, Togarrati PP, Suknuntha K, Kumar A, Samarjeet F, Probasco MD, Tian S, Stewart R, Thomson JA, Slukvin II |
Citation(s) |
22981233 |
Submission date |
Jul 26, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Igor Slukvin |
E-mail(s) |
islukvin@wisc.edu
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Phone |
608-263-0058
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Organization name |
University of Wisconsin
|
Department |
Department of Pathology and Laboratory Medicine
|
Street address |
1220 Capitol Court
|
City |
Madison |
State/province |
WI |
ZIP/Postal code |
53706 |
Country |
USA |
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Platforms (1) |
GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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Samples (17)
|
GSM976973 |
Day 4 differentiated H1 (human embryonic stem cell) derived EMHlin-KDRbrightAPLNR+PDGFRalow/- cells [duplicate_1of2] |
GSM976974 |
Day 4 differentiated H1 derived KDRdim cells |
GSM976975 |
Day 5 differentiated H1 derived VE-cadherin+CD73-CD43+CD235a+CD41a+ cells |
GSM976976 |
Day 5 differentiated H1 derived VE-cadherin+CD73-CD43lowCD235a+CD41a-CD117- cells [duplicate_1of2] |
GSM976977 |
Day 3 differentiated H1 derived endothelial cores from hemangioblast colonies |
GSM976978 |
Day 8 differentiated H1 derived CD34+CD43+CD235a/CD41a+CD45- cells |
GSM976979 |
Day 8 differentiated H1 derived lin-CD34+CD43+CD235a/CD41a-CD45- cells |
GSM976980 |
Day 8 differentiated H1 derived lin-CD34+CD43+CD235a/CD41a-CD45+ cells |
GSM976981 |
Human umbelical vein endothelial cells derived RNA |
GSM976982 |
Day 3 differentiated H1 derived EMHlin-APLNR+PDGFRa+ cells |
GSM976983 |
Day 4 differentiated H1 (human embryonic stem cell) derived EMHlin-KDRbrightAPLNR+PDGFRalow/- cells [duplicate_2of2] |
GSM976984 |
Day 4 differentiated H1 derived KDRbright VE-cadherin+ cells |
GSM976985 |
Day 5 differentiated H1 derived VE-cadherin+CD73-CD235a/CD43-CD117intermediate cells |
GSM976986 |
Day 5 differentiated H1 derived VE-cadherin+CD73-CD43lowCD235a+CD41a-CD117- cells [duplicate_2of2] |
GSM976987 |
Day 5 differentiated H1 derived VE-cadherin+CD73+CD235a/CD43-CD117high cells |
GSM976988 |
H1 derived endothelial cells at passage 2 |
GSM976989 |
Undifferentiated H1 |
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Relations |
BioProject |
PRJNA171560 |
SRA |
SRP014620 |