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Series GSE39707 Query DataSets for GSE39707
Status Public on Jul 28, 2012
Title Zebrafish whole embryos at 36 hpf treated with DMSO or 5uM 11,12-EET
Organism Danio rerio
Experiment type Expression profiling by array
Summary In our previous study, we found zebrafish embryos treated with 5uM 11,12-EET (epoxyeicosatrienoic acid) had increased stem cell marker, runx1, expression in the AGM. EET also induced ectopic runx1 expression in the tail. To systematically study how EET regulates gene expression, we performed microarray analysis on EET-treated embryos.
Zebrafish whole embryos were synchronized at fertilization. Embryos were grown at 28 degree overnight. 25 embryos per group were treated with DMSO or 5uM 11,12-EET starting from 24 hpf (hour post fertilization) until 36 hpf at 28 degree. The triplicates were from three different clutches of embryos, and split into DMSO v.s. EET for each clutch.
Overall design EET vs. DMSO
Contributor(s) Li P, Zhou Y, DiBiase A
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Submission date Jul 27, 2012
Last update date Jan 25, 2018
Contact name Anthony Dominc DiBiase
Organization name Children's Hospital Boston
Department Hem/Onc
Lab Zon Lab
Street address KFRB, 1 Blackfan St, Suite 8216
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM977997 zebrafish embryo at 36 hpf DMSO, biological rep1
GSM977998 zebrafish embryo at 36 hpf DMSO, biological rep2
GSM977999 zebrafish embryo at 36 hpf DMSO, biological rep3
BioProject PRJNA171510

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39707_RAW.tar 10.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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