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Series GSE39731 Query DataSets for GSE39731
Status Public on Jul 17, 2013
Title Expression arrays of KRASG12D rhabdomyosarcoma models in zebrafish
Organism Danio rerio
Experiment type Expression profiling by array
Summary Rhabdomyosarcoma is a pediatric malignancy thought to arise from the uncontrolled proliferation of myogenic cells. Here, we have generated models of rhabdomyosarcoma in the zebrafish by inducing oncogenic KRASG12D expression at different stages during muscle development. Several zebrafish promoters were used including the cdh15 and rag2 promoters that drive gene expression in early muscle progenitors, and the mylz2 promoter that expresses in differentiating myoblasts. The tumors that developed differed in their ability to recapitulate normal myogenesis. cdh15:KRASG12D and rag2:KRASG12D fish developed tumors that displayed an inability to fully undergo muscle differentiation by histologic appearance and gene expression analyses. In contrast, mylz2:KRASG12D tumors more closely resembled mature skeletal muscle and were most similar to well-differentiated human rhabdomyosarcoma by gene expression. mylz2:KRASG12D fish showed significantly improved survival compared to cdh15:KRASG12D and rag2:KRASG12D fish. Tumor-propagating activity was enriched in myf5-expressing cell populations within all of the tumor types. Our results demonstrate that oncogene expression at different stages during muscle development has profound effects on the ability of tumor cells to recapitulate normal myogenesis, altering the tumorigenic capability of these cells.
 
Overall design 32 samples total: 7 WT muscle, 9 mylz2-KRAS, 9 cdh15-KRAS, and 7 rag2-KRAS tumors
 
Contributor(s) Zon L, Storer N, White R
Citation(s) 23821038
Submission date Jul 30, 2012
Last update date Jan 25, 2018
Contact name Richard Mark White
E-mail(s) whiter@mskcc.org
Phone 646-888-3415
Organization name Memorial Sloan Kettering Cancer Center
Department Cancer Biology & Genetics
Lab White Lab
Street address 1275 York Avenue MB 424
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (32)
GSM978304 wild-type muscle_AB12
GSM978305 wild-type muscle_AB2
GSM978306 wild-type muscle_AB5
Relations
BioProject PRJNA171559

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39731_RAW.tar 56.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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