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Series GSE39735 Query DataSets for GSE39735
Status Public on Dec 31, 2013
Title Identification of miR-205 targets using an RIP-Chip assay with AGO2 antibody
Organism Homo sapiens
Experiment type Expression profiling by array
Other
Summary In this study, the prognostic properties of miR-205 expression levels are investigated in a well-documented prostate cancer cohort. We show that miR-205 is correlated to shortened overall survival, significantly dividing the PCa patients into high and low risk groups. Furthermore, miR-205 is shown to inversely correlate to occurrence of metastases. In situ hybridization is also performed, demonstrating high miR-205 expression in the basal cells of benign prostate tissue glands. A RIP-Chip assay using an AGO2 antibody was implemented and the miR-205 targets identified were found to be enriched in MAPK/ERK, Toll-like receptor and IL-6 signaling pathways. We also found individual targets involved in cancer and androgen receptor signaling. Ectopic levels of miR-205 are shown to decrease the level of androgen receptor both at the mRNA and protein levels in prostate cancer cell lines. This is further corroborated in the prostate cancer cohort were miR-205 expression levels in the prostatic tissues are found to inversely correlate to assessment of androgen receptor (AR) immunostaining and to serum levels of PSA, a protein regulated by AR signaling. The level of miR-205 is also found to be significantly lower in castration resistant prostate cancer patients than in hormone naïve patients. Our data indicates that miR-205 is regulated by androgens and act by different mechanisms in androgen depleted settings, e.g. giving opposite effects on adhesion. Taken together these findings imply that miR-205 might have therapeutic potential especially for the castration resistant and currently untreatable form of prostate cancer.
 
Overall design Experiment done with biological triplicates. Three with miR-205 ectopic expression and three with negative control mimic ectopic expression. Followed by a RIP-Chip, ending with mRNA extraction and gene expression array.
 
Contributor(s) Hagman Z, Ceder Y
Citation(s) 23571738
Submission date Jul 30, 2012
Last update date Jul 26, 2018
Contact name Yvonne Ceder
E-mail(s) yvonne.ceder@med.lu.se
Organization name Lund University
Department Laboratory Medicine
Lab TCR
Street address Medicon Village
City Lund
ZIP/Postal code 22381
Country Sweden
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (6)
GSM978367 PC3 cells transfected with miR-205, replicate 1
GSM978368 PC3 cells transfected with miR-205, replicate 2
GSM978369 PC3 cells transfected with miR-205, replicate 3
Relations
BioProject PRJNA171573

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39735_RAW.tar 27.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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