|
| Status |
Public on Apr 10, 2013 |
| Title |
Tfe3-bound regions in naive pluripotent mouse embryonic stem (ES) cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The bHLH transcription factor Tfe3 is a powerful regulator of pluripotency and we report a genome-wide analysis of Tfe3 occupancy in mouse ES cells. Nuclear localization of Tfe3 is inhibited by a protein complex containing the tumor-suppressor Folliculin (Flcn) and we also determine Tfe3 binding sites in ES cells expressing an shRNA targeting Flcn. Specificity is controlled for by using unspecific IgGs and ES cells expressing an shRNA targeting Tfe3.
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| |
|
| Overall design |
ChIP-Seq profiling of Tfe3 in ES cells
|
| |
|
| Contributor(s) |
Betschinger J, Nichols J, Corrin PD, Paddison PJ, Smith A |
| Citation(s) |
23582324 |
| Submission date |
Aug 01, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Joerg Betschinger |
| E-mail(s) |
jb579@cam.ac.uk
|
| Organization name |
Wellcome Trust and Medical Research Council Stem Cell Institute
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QR |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA171828 |
| SRA |
SRP014667 |
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