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Series GSE39830 Query DataSets for GSE39830
Status Public on Dec 01, 2019
Title Milk- and soy-based diets have differential effects on colitis and colon gene expression in the Il10-/- mouse
Organism Mus musculus
Experiment type Expression profiling by array
Summary Milk and soy are reported to contain bioactive molecules with antibacterial and immunomodulatory actions, which may be beneficial to people with IBD. The aim of this study was to determine whether diets containing ruminant milk or soy solids reduce intestinal inflammation in Il10-/- mice. Male Il10-/- mice and C57BL/6J mice were fed diets containing 40% (w/w) sheep, goat, or cow whole milk powder, 40% (w/w) soy solids (NOW® Foods Soy Milk Powder, Instant), or one of two control diets (casein-free modified-AIN76A or standard AIN76A) from 4 to 11 weeks of age. Diets were based on AIN76A, which was included as an inter-experimental control for inflammation. For all diets except AIN76A, total protein, fat, carbohydrate and energy were kept as similar as possible. Weight and food intake were measured throughout the experiment (three times weekly), and intestinal tissue was taken for histopathology evaluation of inflammation and analysis of gene expression.
Analysis of mouse weight and feed intake both showed a significant strain-diet interaction: Il10-/- mice fed the cow and goat milk diets ate less and gained less weight than all the other diet groups. This diet effect was not evident for the C57BL/6J mice. Il10-/- mice on the cow and goat milk diets had reduced colon histological injury scores relative to those on the other diets. Il10-/- mice on the cow and goat milk diets also had reduced expression of many immune/inflammatory-related genes and pathways.
 
Overall design 6x2 factorial design. Two strains of mouse (Il10 knockout mouse and the background strain C57BL/6J) were sampled at 12 weeks of age after feeding of milk, soy and control diets for 6 weeks, and intact proximal colon and colon epithelium harvested from each mouse (6 mice per group).
 
Contributor(s) Russ AE, Roy NC, Reynolds GW, Anderson RC, Barnett MP, McNabb WC, Young W, MacLean P, Park Z, Ryan L
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Submission date Aug 02, 2012
Last update date Dec 01, 2019
Contact name Anna Moore
E-mail(s) anna.russ@agresearch.co.nz, anna.moore.1983@gmail.com
Organization name AgResearch Ltd
Street address Tennent Drive
City Palmerston North
ZIP/Postal code 4412
Country New Zealand
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (72)
GSM979930 Colon_C57_Goat milk_High [US81303224_251486828629_S01_GE2-v5_95_Feb07_1_1]
GSM979931 Colon_IL10_Soy_High [US81303224_251486828629_S01_GE2-v5_95_Feb07_1_2]
GSM979932 Colon_IL10_ModifiedAIN76A_Low [US81303224_251486828629_S01_GE2-v5_95_Feb07_1_3]
Relations
BioProject PRJNA171997

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39830_RAW.tar 1.1 Gb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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