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| Status |
Public on Aug 30, 2012 |
| Title |
Bromodomain-dependent stage-specific male genome programming by Brdt [Illumina BeadArray] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Male germ cell differentiation is a highly regulated multistep process initiated by the commitment of progenitor cells into meiosis and characterized by major chromatin reorganizations in haploid spermatids. We report here that a single member of the double bromodomain BET factors, Brdt, is a master regulator of both meiotic divisions and post-meiotic genome repackaging. Upon its activation at the onset of meiosis, Brdt drives and determines the developmental timing of a testis-specific gene expression program. In meiotic cells, Brdt initiates a genuine histone acetylation-guided programming of the genome by activating essential meiotic genes and repressing a “progenitor cells” gene expression program, while “priming” a post-meiotic gene group for further activation. At post-meiotic stages, a global chromatin hyperacetylation gives the signal for Brdt’s first bromodomain to direct the genome-wide replacement of histones by transition proteins. Brdt is therefore a unique and essential regulator of male germ cell differentiation, which, by using various domains in a developmentally controlled manner, first drives a specific spermatogenic gene expression program, and later controls the tight packaging of the male genome.
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| Overall design |
Total RNA obtained from testes from prepuberal mice at 17dpp and at 20dpp were compared between Brdt-/- and Brdt+/- (17dpp), between Brdt-/- and Brdt +/+ (20dpp) and between BrdtBD1del and Brdt wt mice (20dpp). In each experiments, 6 replicates of each genotype and condition were used.
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| Contributor(s) |
Rousseaux S, Khochbin S, Gaucher J, Guardiola P |
| Citation(s) |
22922464 |
| Submission date |
Aug 06, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Sophie Rousseaux |
| Organization name |
INSERM-UGA
|
| Department |
IAB
|
| Lab |
U1209
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| Street address |
Bd de la Chantourne
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| City |
La Tronche |
| ZIP/Postal code |
38706 |
| Country |
France |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE39910 |
Bromodomain-dependent stage-specific male genome programming by Brdt |
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| Relations |
| BioProject |
PRJNA172755 |
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