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Series GSE39967 Query DataSets for GSE39967
Status Public on Aug 09, 2012
Title Transcriptional profiles of unfractionated lymph nodes cells obtained from completely protected, non protected and unvaccinated control rhesus macaques seven days prior to, and four and fourteen days after wt SIVmac239 challenge by microarray analysis.
Platform organism Homo sapiens
Sample organism Macaca mulatta
Experiment type Expression profiling by array
Summary Live-attenuated SIV vaccines (LAVs) remain the most efficacious of all vaccines in nonhuman primate models of HIV/AIDS, yet the basis of their robust protection remains poorly understood. Here, we demonstrate that the degree of LAV-mediated protection against intravenous (IV) wildtype (wt) SIVmac239 challenge strongly correlates with the magnitude and function of SIV-specific, effector-differentiated T cells in lymph node (LN), but not with such T cell responses in blood or with other cellular, humoral and innate immune parameters. Maintenance of protective T cell responses was associated with persistent LAV replication in LN, which occurred almost exclusively in follicular helper T cells. Thus, effective LAVs maintain lymphoid tissue-based, effector-differentiated, SIV-specific T cells that intercept and suppress early wt SIV amplification and, if present in sufficient frequencies, can completely control and perhaps clear infection -- an observation that provides rationale for development of safe, persistent vectors that can elicit and maintain such responses.
 
Overall design There are four protection outcome groups: unvaccinated control (C), complete protect (CP), non-protect (NP) and partial protect (PP). Respectively for these groups, there are 7, 9, 6 and 4 animals sampled seven days pre-challenge (minus7PCD); 9, 13, 5 and 5 animals sampled four days post-challenge (4PCD) and 8, 11, 5 and 3 fourteen days post-challenge (14PCD).
 
Contributor(s) Fukazawa Y, Park H, Cameron MJ, Lefebvre F, Lum R, Coombes N, Mahyari E, Hagen S, Bae JY, Delos Reyes M, Swanson T, Legasse AW, Sylwester A, Hansen SG, Smith AT, Stafova P, Shoemaker R, Li Y, Oswald K, Axthelm MK, McDermott A, Ferrari G, Montefiori DC, Edlefsen PT, Piatak M, Lifson JD, Sékaly RP, Picker LJ, Wilkinson PA
Citation(s) 22961108
Submission date Aug 08, 2012
Last update date Mar 20, 2017
Contact name Peter A Wilkinson
Organization name Case Western Reserve University
Department Pathology / Systems Biology & Bioinformatics
Street address 2103 Cornell Road
City Cleveland
State/province Ohio
ZIP/Postal code 44120
Country USA
 
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (85)
GSM982344 CP_4PCD_Rh23841
GSM982345 C_4PCD_Rh24062
GSM982346 C_4PCD_Rh25430
This SubSeries is part of SuperSeries:
GSE40006 Lymph node T cell responses predict the efficacy of live attenuated SIV vaccines
Relations
BioProject PRJNA172175

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39967_RAW.tar 3.9 Mb (http)(custom) TAR
GSE39967_non-normalized_LN.txt.gz 7.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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